氨氯地平对兔动脉粥样硬化及炎症因子妊娠相关血浆蛋白A作用的影响  

Effects of Amlodipine on Atherosclerosis and Inflammatory Marker Pregnancy-associated Plasma Protein-A in Rabbits

作　　者：李璇[1] 陈良[1] 张梅[1] 李长江[1] 萧亚伟[1] 李晓楠[1] 徐新生[1] 蒋桂花[1] 

机构地区：[1]教育部和卫生部心血管重构和功能研究重点实验室山东大学齐鲁医院心内科,山东省济南市250012

出　　处：《中国动脉硬化杂志》2009年第6期444-448,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的观察氨氯地平对动脉粥样硬化炎症因子妊娠相关血浆蛋白A的作用。方法20只雄性新西兰大白兔应用球囊拉伤腹主动脉后高脂饲养12周,然后随机分为氨氯地平组和对照组,分别给予氨氯地平5 mg/d和溶媒对照治疗,治疗12周后处死动物;在实验开始,12周末和处死前耳缘静脉抽血检测高敏C反应蛋白和妊娠相关血浆蛋白A的水平,同时应用高频超声检测腹主动脉;处死动物后留取腹主动脉,分别进行常规病理检查,免疫组织化学染色检测妊娠相关血浆蛋白A的分布。结果病理学和电镜结果显示,与对照组比较氨氯地平组斑块明显减小、脂质含量降低、平滑肌纤维较多而胶原少;超声检查也显示氨氯地平组内膜-中层厚度、收缩末期直径均明显减小(P<0.01),而校正的内中膜回声强度明显增加(P<0.01)。氨氯地平还可以明显降低血清妊娠相关血浆蛋白A和高教C反应蛋白的水平(P<0.01);免疫组织化学显示氨氯地平组斑块局部妊娠相关血浆蛋白A的表达减少;且血清妊娠相关血浆蛋白A的水平与高敏C反应蛋白和内膜-中层厚度相关(R^2=0.424和R^2=0.339)。结论妊娠相关血浆蛋白A与动脉粥样硬化的严重程度密切相关,氨氯地平可以抑制妊娠相关血浆蛋白A,从而延缓动脉粥样硬化的进展。Aim To observe the effect of amlodepine on pregnancy-associated plasma protein-A in atherosclerosis. Methods 20 male New Zealand rabbits were fed on a diet of 1% cholesterol for 12 weeks after they underwent ballon-induced abdominal arotic wall injury. At the end of the 12th week, they were divided into 2 groups at random. One group was given Amlodipine （5 mg/d） for 12 weeks, the other was control group. At the beginning of the experiment, the end of 12th week and 24th week, blood samples were collected to measure the concentrations of pregnancy-associated plasma protein-A and high sensitive C-reaction protein; at the same time, abdominal aorta were examined by high frequency ultrasound. At the end of 24th week, abdominal aorta were processed and examined by pathological stain and immunohistochemieal stain for pregnancy-associated plasma protein-A. Results Pathological results and electron microscope showed that compared with control group, the plaque area and Lipid deposition of amlodipine group reduced, smooth muscle cells increased and collagen minished. Ultrasound measurements also showed that intima-media thickness and end systolic diameter were significantly decreased （P 〈0. 01 ） and corrected intima-media echo intensity was increased （P 〈 0.01 ） in amlodipine group. Amlodipine also decreased pregnancy-associated plasma protein-A and high sensitive C-reaction protein in blood （P 〈 0.01 ） and pregnancy-associated plasma protein-A expression in atheroscleretic plaque with immunohistochemical stain. Pregnancy-associated plasma protein-A had a close relationship with high sensitive C-reaction protein and intima-media thickness （R^2 = 0. 424 and R^2 = 0. 339）. Conclusion Pregnancy-associated plasma protein-A is associated with severity of atherosclerosis. Amledipine can regress atherosclerosis by inhibiting pregnancy-associated plasma protein-A.

关 键 词：动脉粥样硬化 腹主动脉 妊娠相关血浆蛋白A 高敏C反应蛋白 超声检查 氨氯地平 

分 类 号：R363[医药卫生—病理学]