羊布鲁氏菌的分泌蛋白质组分析  

Proteome Analysis of Secreted Proteins of Brucella melitensis

作　　者：王玉飞[1] 曲勍[1,2] 乔凤[1,2] 钟志军[1] 杜昕颖[1] 汪舟佳[1] 陈泽良[1] 黄留玉[1] 

机构地区：[1]军事医学科学院疾病预防控制所,北京100071 [2]吉林大学公共卫生学院人畜共患病教育部重点实验室,吉林长春130021

出　　处：《微生物学通报》2009年第8期1150-1157,共8页Microbiology China

基　　金：国家"十一五"重大专项子课题(No.2008ZX1004-015);国家863计划项目(No.2007AA02Z412)

摘　　要：分泌蛋白是指那些分泌到细胞外的蛋白质。布鲁氏菌的分泌蛋白可能介导了病原与宿主之间的相互作用,在布鲁氏菌的毒力方面发挥一定的作用,但是研究方法的局限性限制了分泌蛋白的研究。本文报道了利用蛋白质组的方法来寻找羊布鲁氏菌的分泌蛋白。首先用TCA-丙酮法提取布鲁氏菌培养上清中的分泌蛋白,双向电泳进行分离,然后用质谱来鉴定这些蛋白,最终鉴定到40种蛋白。通过生物信息学分析,发现这些蛋白主要是ABC转运系统的底物结合蛋白、外膜蛋白和热休克蛋白。这些蛋白的识别不仅有助于对布鲁氏菌致病机制的理解,而且也可为布鲁氏菌病的疫苗研制提供靶标蛋白。Secreted proteins （the secretome） of the Brucella may mediate important pathogen-host interactions, but such proteins are technically difficult to analyze. In the present study, we describe an approach to identify Brucella melitensis secreted proteins by using proteome analysis. TCA-acetone was used to recovcry extracellular proteins from the culture of Brucella rnelitensis, and two-dimensional gel electrophoresis followed by mass spectrometry for protein resolution and identification. At last, 40 proteins were identified. These proteins included substrate-binding proteins of ABC transporters, outermembrane proteins and heat shock proteins. The identification of these secreted proteins provides important clues for the understanding of the molecular mechanism of Brucella and interesting candidates for the vaccine to treat or prevent Brucella infections.

关 键 词：布鲁氏菌 分泌蛋白 双向电泳 质谱 

分 类 号：S852.61[农业科学—基础兽医学]