偏二甲基肼诱发中国仓鼠肺细胞有丝分裂纺锤体损伤  

Mitotic spindle damage induced by unsymmetrical dimethyl hydrazinein Chinese hamster fibroblast cells

作　　者：王琼[1] 高沛永[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《中国药理学与毒理学杂志》1998年第3期239-240,共2页Chinese Journal of Pharmacology and Toxicology

摘　　要：偏二甲基肼诱发中国仓鼠肺细胞有丝分裂纺锤体损伤王琼高沛永（军事医学科学院放射医学研究所，北京１００８５０）偏二甲基肼（ｕｎｓｙｍｍｅｔｒｉｃａｌｄｉｍｅｔｈｙｌｈｙｄｒａｚｉｎｅ，ＵＤＭＨ）的主要用途是作火箭推进剂，对其致癌作用已有不少报道，但对其致...By using differential staining technique for simultaneous visualization of mitotic spindle and chromosomes, the mitotic spindle damage induced by unsymmetrical dimethyl hydrazine (UDMH) in Chinese hamster fibroblast cell line (CHL) was studied. Experimental results showed that with increasing UDMH concentrations the number of metaphase with normal morphology of spindles decreased obviously. When the concentration of UDMH reached 348 mg·L1, normal spindle could not been seen. At the concentration less than 232 mg·L1 of UDMH, various types of abnormal spindle damage could be observed. There were two kinds of abnormal spindle metaphase with higher incidence, of which one is noncongression and another is apolar (ball metaphase). The incidence of ball metaphase was 100% at the highest UDMH concentration. It is likely that spindle damage by UDMH during mitosis is related to carcinogenicity of UDMH.

关 键 词：偏二甲基肼 CHL 肺细胞 有丝分裂 纺锤体 诱变性 

分 类 号：R992[医药卫生—毒理学]