空肠弯曲菌Cj1136、Cj1138、Cj1139基因对比研究  

Comparison of Cj1136, Cj1138 and Cj1139 genes among Campylobacterjejuni strains

作　　者：李鑫[1] 李震中[1] 白欣立[2] 刘慧[3] 赵子春[1] 刘卫卫[1] 高爱民[1] 邢丛丛[1] 李春岩[1] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050000 [2]河北医科大学第二医院儿科,石家庄050000 [3]中国医学科学院北京协和医院神经内科

出　　处：《中华流行病学杂志》2009年第8期829-831,共3页Chinese Journal of Epidemiology

基　　金：国家“863”高技术研究发展计划（2006AA02A237）;国家自然科学基金（30471919）

摘　　要：目的了解吉兰-巴雷综合征（GBS）相关空肠弯曲菌（C．jejuni）的Cj1136、Cj1138、Cj1139基因序列特征，并同GenBank中的非GBS相关C．jejuni菌株对应序列进行比较，找出可能使C.jejuni菌株具有致GBS能力的碱基突变。方法选取分离自GBS患者粪便并经动物模型证实为致GBS的AMAN型C.jejuni 3株进行培养并提取基因组DNA测序。将基因测序结果与NCTC11168菌株进行对照比较，寻找此3株C.jejuni的Cj1136、Cj1138、Cj1139基因突变位点，并计算此3株致GBS C. jejuni的Cj1136、Cj1138、Cj1139基因序列间的遗传距离。结果3株致GBS C．jejuni菌株的Cj1136基因由1173个碱基构成；Cj1138基因由1170个碱基构成；Cj1139基因由912个碱基构成，与NCTC11168的Cj1136、Cj1138、Cj1139基因序列相比，此3株致GBS C. jejuni中只有Cj1138存在2个相同的碱基突变。遗传距离计算，3株致GBS C．jejuni的Cj1136、Cj1138、Cj1139基因之间最大遗传距离为2．1％。结论与NCTC11168相比GBS相关C.jejuni中Cj1138基因序列存在相同碱基突变，这些碱基突变可能与C.jejuni致GBS能力的改变相关。该3株致GBS C.jejuni的Cj1136、Cj1138、Cj1139基因遗传距离较小，反映河北地区致GBS的C.jejuni在进化上存在聚类现象，具有一定的区域特征。Objective By sequenceing the Cj1136, Cj1138 and Cj1139 gene of Campylobacter jejuni （C. jejuni） strains associated with Guillain-Barr6 Syndrome （GBS）, features of Cj1136, Cj1138 and Cjl 139 gene were studied. Results were compared with the C. jejuni strain NCTC 11168, to find the mutations in sequence of C. jejuni which inducing GBS and their polygenetic relationship was analyzed. Methods Three GBS-associated C. jejuni strains were isolated from stools of GBS patients from Hebei province who had been diagnosed as clinical AMAN pattern and electrophysiological tests were performed. After distilling and sequencing Cj1136, Cj1138 and Cj1139 genes, results were spliced and assembled into a complete sequence by the terminals overlapped with each other. Sequences of Cj1136, Cj1138 and Cj1139 genes were compared with NCTC11168, to find the mutations and gene feature. Results The CJ1136, Cj1138 and Cj1139 gene of the three GBS- associated C. jejuni strains were composed by 1173 base pairs, 1170 base pairs, 912 base pairs respectively. The alignment with the related sequence of NCTC11168 showed that there were two same mutations in the Cj1138 gene of the three C. jejuni stains. Data from phylogenetic analysis demonstrated that the three C. jejuni strains were genetically closed to NCTC11168, with the biggest phylogenetic distance between the three of them as 2.1%. Conclusion When compared with NCTC11168 the Cj1138 gene of the three GBS-associated C. jejuni strains had the same mutations which might be related to the development of GBS. Relation between the variation and GBS-pathogenesis remained to be confirmed. The mutations found in the three C. jejuni strains established the foundation for exploring the biological characteristics of GBS-associated C. jejuni strains and demonstrated that the GBS- associated C. jejuni strains of Hebei province having its regional features.

关 键 词：吉兰-巴雷综合征 空肠弯曲菌 基因 序列分析 

分 类 号：R745.43[医药卫生—神经病学与精神病学] R373.21[医药卫生—临床医学]