酸枣仁乳饮料中有效成分酸枣仁皂苷A的含量测定方法研究  

Research on determination of Jujuboside A in Semen Ziziphi spinosae milk drink

作　　者：刘魁[1] 戎欣玉[2] 王奎涛[2] 王云清[1] 李子成[1] 

机构地区：[1]河北科技大学理学院,石家庄050018 [2]河北科技大学化学与制药工程学院,石家庄050018

出　　处：《食品科技》2009年第8期264-266,共3页Food Science and Technology

基　　金：河北省教育厅项目(2006316)

摘　　要：建立了酸枣仁乳饮料中主要有效成分酸枣仁皂苷A的含量测定方法。采用Kromasil ODS-1 C18色谱柱(250mm×4.6mm,5μm);以乙腈-水=34∶66(v/v)为流动相,检测波长为204nm。酸枣仁皂苷A在0.070～0.70mg/mL范围内与峰面积(Y)呈良好的线性关系(r=0.999),样品溶液至少在8h内稳定,回收率为94.1%,RSD为1.96%。本方法简便、快速,专属性好,结果可靠,可用于该产品的质量控制。A method for determination of Jujuboside A in Semen Ziziphi spinosae milk drink by RP-HPLC was established. The column is Kromasil ODS-1 C18（250 mm×4.6 mm, 5 μm）. The mobile phase is acetonitrile- water （34：66）. The UV detector is set at 204 nm. The linear range of the determination is 0.070-0.70 mg/mL with the correlation coefficient of 0.999. The sample solution is stable within 8 h. The average recovery rate was 94.1% with RSD of 1.96%. The method is simple, accurate and specific. It is suitable for the determination of Jujuboside A in Semen Ziziphi spinosae milk drink.

关 键 词：酸枣仁 饮料 酸枣仁皂苷A 反相高效液相色谱法 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]