大孔树脂纯化长白楤木叶皂苷及其生物活性研究  

Purification of Saponins from Aralia continentalis Leaves Using Macroporous Adsorption Resin and Its Bioactivity

作　　者：冯颖[1,2] 李天来[2] 孟宪军[1] 范文丽[2] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110161 [2]沈阳农业大学园艺学院,辽宁沈阳110161

出　　处：《食品科学》2009年第16期158-161,共4页Food Science

基　　金：中国博士后科学基金资助项目(20080431155)

摘　　要：通过静态吸附与解吸实验对4种大孔树脂进行筛选。结果表明,HPD-100树脂对长白楤木叶皂苷的吸附和解吸效果最好,宜用于纯化该皂苷;动态吸附解吸实验表明,HPD-100树脂纯化长白楤木叶皂苷的最佳条件为:上柱液皂苷浓度0.3mg/ml,上柱液体积18BV,上柱液pH5,梯度洗脱条件为水→10%乙醇→30%乙醇→50%乙醇;清除超氧阴离子自由基和抑菌实验结果表明,30%和50%乙醇梯度洗脱所得纯化皂苷样品均具有一定的清除超氧阴离子自由基和抑菌的活性。Static absorption and desorption experiments were carried out to screen the optimum resin from 4 types of macroporous adsorption resins and macroporous resin HPD-100 was found to have the best performance for the purification of crude extract of saponins from Aralia continentalis leaves. In dynamic adsorption and desorption experiments, the optimum purification conditions of saponins by HPD-100 were determined as follows： saponin concentration 0.3 mg/ml, sampling volume 18 times of bed volume （BV）, pH value of the sample 5, and gradient elution water → 10% ethanol → 30% ethanol→ 50% ethanol. The eluates of 30% and 50% ethanol both had certain scavenging activity to superoxide radical and antibacterial activity.

关 键 词：长白楤木 皂苷 大孔树脂 

分 类 号：R284.1[医药卫生—中药学] TQ028.15[医药卫生—中医学]