哈密瓜ACC合成酶基因cDNA的克隆及全序列分析  

Cloning and Sequence Analysis of cDNA Encoding for ACC Synthase from Hami Melon

作　　者：丁群英[1,2] 张瑞 廖新福 郭蔼光[1,2] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学陕西省农业分子生物学实验室,陕西杨凌712100 [3]新疆维吾尔自治区葡萄瓜果开发研究中心,新疆鄯善838201

出　　处：《园艺学报》2009年第8期1177-1183,共7页Acta Horticulturae Sinica

基　　金：新疆维吾尔自治区自然科学基金项目(200721106)

摘　　要：以新疆哈密瓜(Cucumis melo L.)幼叶为材料,根据已报道的甜瓜ACC合成酶3(1-aminocy-clopropane-1-carboxylic acid synthase,ACS)基因片段设计特异引物,采用RT-PCR和RACE(Rapid Amplifi-cation cDNA Ends,cDNA末端快速扩增)等技术,克隆得到编码哈密瓜CMe-ACS3基因的全长cDNA序列,长1895bp,编码482个氨基酸,分子量约为54.12kD,等电点8.45,GenBank登录号为FJ383171。利用软件MEGA4构建进化树,发现哈密瓜ACS3基因与黄瓜中的同类基因亲缘关系最近。哈密瓜CMe-ACS3基因的克隆及序列分析为认识此基因在甜瓜中的功能研究奠定基础。Specific primers were designed based on the reported partial sequence of melon （ Cucumis melo L. ） ACS3 gene in the GenBank to clone the full-length cDNA of ACS3 gene in using RT-PCR and RACE methods. Its GenBank Accession No. is FJ383171. The length of Hami melon ACS gene cDNA is 1 895 bp, this gene encodes 482 amino acids, the putative molecular weight is 54. 12 kD and its pI is 8.45. The phylogenetic trees constructed by software MEGA4 showed that ACS3 gene of hami melon was closely related to that of Cucumis sativus. The cloning of ACS3 gene full-length cDNA from the Hami melon provides the basis for understanding the role of ACS3 gene in melon.

关 键 词：哈密瓜 ACC合成酶 RACE 序列分析 

分 类 号：S652.1[农业科学—果树学] Q781[农业科学—园艺学]