着丝粒蛋白-H对舌鳞癌细胞增殖促进作用的研究  

The research on centromere protein H promoting TSCC cells proliferation

作　　者：武东辉[1,2] 李劲松[1] 林钊宇[1] 陈伟良[1] 潘朝斌[1] 廖文婷[3] 俞春萍[3] 孔庆丽[3] 宋立兵[3] 

机构地区：[1]中山大学附属第二医院口腔颌面外科,广州510120 [2]广州市海珠区口腔医院 [3]中山大学肿瘤防治中心华南肿瘤国家重点实验室

出　　处：《中华口腔医学研究杂志（电子版）》2009年第4期10-13,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省自然科学基金(9151008901000201);广州市科技计划项目(2008Z1-E201)

摘　　要：目的探讨导致恶性肿瘤出现细胞染色体非整倍数现象的着丝粒蛋白-H(CENP-H)在舌鳞癌中的表达情况,及其对舌鳞癌细胞增殖的影响。方法舌鳞状细胞癌组织及其癌旁舌黏膜组织组成配对子,采用RT-PCR、Western blot分别检测8对配对子的舌鳞癌系TSCCa、Tca8113及原代培养的舌黏膜细胞中CENP-HmRNA水平和蛋白的表达水平;采用免疫组织化学SP法检测同样8对配对子舌黏膜中CENP-H的表达;采用MTT法、克隆形成实验法分析转染CENP-H对TSCCa细胞增殖能力的影响。结果舌鳞状细胞癌中CENP-H在mRNA、蛋白质水平的表达高于舌黏膜上皮,且差异有统计学意义;免疫组化显示CENP-H高表达,且阳性染色定位于肿瘤细胞的细胞核,而在舌黏膜鳞状上皮细胞中未见表达;MTT法、克隆形成试验显示转染CENP-H后舌鳞癌细胞增殖能力明显高于未转染的对照组细胞(P<0.001)。结论舌鳞癌细胞中高表达的CENP-H对舌鳞癌细胞增殖起到促进作用,在舌鳞癌的发生发展中可能扮演重要角色。Objective To investigate the expression pattern and promoting proliferation function of centromere protein H （CENP-H） in Squamous cell carcinoma of the tongue which has been demonstrated to be associated with aneuploidy in carcinoma cell. Methods Reverse transcriptase polymerase chain reaction （RT-PCR） and Western blot analysis were performed to test the expression of CENP-H in eight matched pairs of Squamous cell carcinoma of the tongue tissues （C） and adjacent noncancerous tongue tissues （N）, as well as Squamous cell carcinoma of the tongue （TSCC） cell lines and tongue epithelial cells （TEC）, the level of CENP-H in 8 characterized TSCC tissues was determined by immunohistochemistry. MTT assay, colony formation assay displayed that TSCCa with high expression of CENP-H reproductive activity. Results The mRNA and protein level of CENP-H was much higher in cancer tissues （C） and TSCC cell lines than that in adjacent noncancerous tongue tissues （N） and in normal cells, respectively. MTT assay, colony formation assay displayed that high expression of CENP-H promoted the proliferation of TSCC cell line TSCCa （P 〈 0.001）. Conclusion CENP-H is highly expressed in TSCC, and its high expression promotes proliferation.

关 键 词：舌鳞状细胞癌 着丝粒蛋白-H 增殖 

分 类 号：R739.8[医药卫生—肿瘤]