细胞骨架完整性在流体剪切力诱导成骨细胞COX-2基因表达中的作用  

Effect of cytoskeleton integrity on the expression of COX-2 gene in osteoblasts induced by fluid shear stress

作　　者：吴昌敬[1,2] 李友瑞[1] 徐亚娟[1] 郭玲[1] 张艺平[1] 付强[1] 

机构地区：[1]中山大学光华口腔医学院.附属口腔医院.口腔医学研究所,广州510055 [2]广州市中西医结合医院口腔科

出　　处：《中华口腔医学研究杂志（电子版）》2009年第4期34-38,共5页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省科技计划项目(2008B030301121);广东省医学科研基金(A2008227)

摘　　要：目的探讨细胞骨架完整性在流体剪切力诱导成骨细胞COX-2基因表达中的作用。方法原代培养BALB/c小鼠颅骨成骨细胞,采用细胞松弛素D(CD)破坏细胞骨架完整性;以12dyne/cm2的流体剪切力对成骨细胞加载1.5h;分别应用实时荧光定量巢式PCR和免疫荧光的方法检测COX-2基因mRNA和蛋白的表达水平,并对结果进行双因素方差分析。结果采用CD破坏细胞骨架完整性,可以对流体剪切力诱导成骨细胞COX-2mRNA和蛋白的表达起拮抗作用(P<0.05);在无流体剪切力加载条件下,CD处理对COX-2mRNA和蛋白的表达水平无显著影响(P>0.05);流体剪切力加载1.5h能使成骨细胞COX-2mRNA和蛋白的表达水平显著升高(P<0.05);CD处理可显著降低流体剪切力诱导成骨细胞COX-2mRNA和蛋白的表达水平(P<0.05)。结论保持细胞骨架完整性是流体剪切力诱导成骨细胞COX-2基因表达过程中所必需的。Objective To study the effect of cytoskeleton integrity on the expression of COX-2 gene in osteoblasts induced by fluid shear stress. Methods BALB/c mouse primary osteoblasts were isolated by enzyme digestion technique. Osteoblasts were treated with or without Cytochalasin D and then loaded or unloaded by fluid shear stress at 12 dyne/cm2. The nested real-time PCR and immunofluorescence were performed to detect the expression levels of COX-2 mRNA and protein in the osteoblasts, respectively. Statistical analyses were performed using two-way ANOVA. Results Cytoskeleton integrity disruption with Cytochalasin D had antagonistic effect on the expression of COX-2 gene induced by fluid shear stress （P 〈 0.05）. Cytoehalasin D had no significant effects on the expression levels of COX-2 mRNA and protein under unloaded condition （P 〉 0.05）. Fluid shear stress significantly increased the expression levels of COX-2 mRNA and protein in osteoblasts （P 〈 0.05）. Cytochalasin D significantly decreased the expression levels of COX-2 mRNA and protein in osteoblasts induced by fluid shear stress （P 〈 0.05）. Conclusion Cytoskeleton integrity in osteoblasts is essential for the expression of COX-2 gene induced by fluid shear stress.

关 键 词：COX-2基因 流体剪切力 细胞骨架 成骨细胞 

分 类 号：R78[医药卫生—口腔医学]