液相色谱法对染发剂中5种n-氨基n--硝基苯酚同分异构体的同时测定  

Simultaneous Determination of Five n-Amino-n-nitrophenols Isomers in Hair Dye of Oxidising Colouring Agents by High Performance Liquid Chromatography

作　　者：于文莲[1] 陈伟[1] 周新[1] 王立峰[1] 孙鑫[1] 王 陈会明[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123

出　　处：《分析测试学报》2009年第8期975-977,共3页Journal of Instrumental Analysis

基　　金：国家质检总局科研项目资助(2007IK097)

摘　　要：建立了同时检测染发剂中5种氨基硝基苯酚氧化剂的高效液相色谱分析方法。实验在避光条件下进行，样品用甲醇溶解后超声提取，采用新型ZORBAX Eclipse Plus C18色谱柱，以甲醇、乙腈、25mmol／L磷酸二氢钾缓冲溶液（pH7．0）为流动相梯度洗脱，二极管阵列检测器检测，检测波长为225nm。在15min内将5种n-氨基-n-硝基苯酚同分异构体分离测定，在0．1～100mg／L范围内线性良好，相关系数为0．998～0．999，定量下限为0．1—0．2mg／L。该方法可同时满足进出口染发剂中5种氧化剂的检验需要。A high performance liquid chromatography（HPLC） method was proposed for simultaneous determination of five n-amino-n-nitrophenols in hair dye under dark conditions. The samples were dissolved by methanol and extracted by ultrasonic extraction. The separation of analytes was performed on a new ZORBAX Eclipse Plus C18 column by a gradient elution using methanol, acetonitrile and 25 mmol/L potassium dihydrogen phosphate solution （pH = 7.0） as ternary mobile phase. The analytes were detected by a photo-diode array detector at 225 nm. The result indicated that the five isomers were completely separated in 15 min. The calibration curves showed good linearity in the range of 0. 1 - 100 mg/L with correlation coefficients of 0. 998 - 0. 999. The quantification limits ranged from 0. 1 to 0. 2 mg/L. The method could meet the requirements for the simultaneous determination of 5 oxidising colouring agents in hair dye -for import and export inspection.

关 键 词：高效液相色谱 n-氨基-n-硝基苯酚 同分异构体 染发剂 

分 类 号：O657.72[理学—分析化学] TQ658.34[理学—化学]