T细胞亚群、NK细胞活性及Th_1/Th_2细胞因子漂移在FOLFOX化疗前后的动态变化  

Study on T lymphocyte sub-clusters,NK activity and Th_1/Th_2 cytokine drift during FOLFOX chemotherapy

作　　者：陈灵芝[1] 康云平[2] 王雪春[1] 黄常新[2] 

机构地区：[1]浙江省嘉兴市第一医院,浙江嘉兴314000 [2]浙江省杭州市第三人民医院,浙江杭州310009

出　　处：《现代实用医学》2009年第7期688-690,共3页Modern Practical Medicine

基　　金：国家自然科学基金项目;编号 30872380;浙江省科技攻关项目;编号 2006C33023

摘　　要：目的研究FOLFOX化疗对肠癌根治性术后患者免疫功能的影响。方法83例肠癌患者根治性术后6周取外周静脉血,采用抗体标记流式细胞仪检测T细胞亚群细胞,MTT法测NK细胞活性,酶联免疫吸附法(ELISA)检测血清中Th1/Th2细胞因子。1个月为1个疗程,共化疗6个疗程。分别于化疗开始前、化疗结束时和化疗结束后6个月监测外周血CD3+T、CD4+T和CD8+T数量比例,NK细胞活性,以及血清中白细胞介素-2(IL-2)、干扰素-(IFN-)、白细胞介素-4(IL-4)、白细胞介素-10(IL-10)的动态水平变化。结果CD8+T、CD4+T/CD8+T化疗后明显下降(均<0.05),化疗结束后6个月恢复至化疗前水平。化疗组的NK细胞活性在化疗结束时较对照组和自身化疗前低(均<0.01);化疗结束后6个月NK细胞活性略低于自身化疗前及对照组同期水平,但差异无显著意义(>0.05)。化疗结束时IFN-、IL-2、IL-4和IL-10水平较对照组或自身化疗前低,化疗结束后6个月恢复正常(均<0.05)。结论FOLFOX化疗可能降低肠癌根治性术后患者的CD8+T的数量比例及NK细胞活性,引起Th1、Th2细胞因子血清水平下降,抑制抗肿瘤免疫功能。Objective To examine the influence of FOLFOX chemotherapy on the immunostatus of the patients with radical cure operation of intestine carcinoma.Methods The peripheral vein blood of 83 cases of the patients was taken as specimens,and T lymphocyte sub-clusters,NK activity and Th1/Th2 cytokines were detected with Fluorescence activated cell sorter（FACS）,MTT assay and enzyme-linked immunosorbent assay（ELISA）respectively.One course of FOLFOX chemotherapy a month was administrated on each patient with six courses consecutive. Before and after the chemotherapy the changes were investigated of interleukin-2（IL-2）, interferon-γ（IFN-γ）, interleukin-4（IL-4）, interleukin-10（IL-10）, NK activities and the percentage of CD3^＋T, CD4^＋T and CD8^＋T.Results CD3^＋ T, CD4^＋T/CD8^＋ T decreased when the chemotherapy finished than before （P 〈 0.05）, and recovered up to the common level six months after the chemotherapy. So did IL-2, IFN-γ, IL-4, IL-10 and NK activity when compared with those before the chemotherapy or the controls （P 〈 0.05）.Conclusion For the patients with radical cure operation of intestine cancer, FOLFOX chemotherapy could decrease CD8^＋T count ratio, NK activity and the cytokine levels of both Th 1 type and th2 type, thereby inhibit the anticancer immune functions of the patients.

关 键 词：药物疗法 免疫组织化学 T细胞亚群、NK细胞活性 TH1/TH2细胞因子 

分 类 号：R730.53[医药卫生—肿瘤] R735.3[医药卫生—临床医学]