脑益康对阿尔茨海默病大鼠tau蛋白过度磷酸化及其相关酶类表达的影响  

Effects of Naoyikang on Tau Hyperphosphorylation and Protein Expression of Its Related Enzymes in Rat’s Brain with Alzheimer’s Disease

作　　者：周静[1] 周爱玲[1] 路燕[1] 

机构地区：[1]南通大学医学院病理生理学教研室,江苏南通226001

出　　处：《苏州大学学报（医学版）》2009年第3期426-429,597,共5页Suzhou University Journal of Medical Science

基　　金：江苏省自然科学基金资助项目(BK2004048);2008年南通市应用研究科技计划项目(K2008009)

摘　　要：目的探讨脑益康对阿尔茨海默病(AD)模型大鼠海马神经元tau蛋白过度磷酸化及其相关酶类表达的影响。方法采用β-淀粉样蛋白1-40(Aβ1-40)右侧海马注射制备AD大鼠模型,免疫组化法和Western blot法检测大鼠海马神经元tau蛋白在Ser404位点的磷酸化水平、蛋白磷酸酯酶2A(PP-2A)的表达情况,ELISA法检测糖原合酶激酶-3β(GSK-3β)蛋白的水平。结果AD模型组大鼠海马组织p-tau(Ser404)、GSK-3β表达增加,PP-2A表达减少,与假手术组的差异有高度统计学意义(P<0.01);脑益康治疗组大鼠的p-tau(Ser404)、GSK-3β表达显著减少,PP-2A表达显著增加,差异均有高度统计学意义(均P<0.01)。结论脑益康可通过增加PP-2A的表达和抑制GSK-3β的表达减轻AD模型大鼠tau蛋白的过度磷酸化。Objective To observe the effects of Naoyikang on tau hyperphosphorylation and protein expression of its related enzymes of Alzheimer＇s disease （AD） rats. Methods AD rat models were established by the injection of Aβ1 -40 into the right hippocampus. The level of phosphorylation of tau protein at the site of Ser404 and the expression of PP -2A in AD rats＇ hippocampus were detected by immunohistochemistry and Western-blot analysis.The expression of GSK-3β was measured by ELISA. Results In AD model group,the expression of p-tau （Ser404）and GSK-3β were increased , whereas the expression of PP-2A was decreased in hippocampus, which were significantly different compared with that in the sham-operated group（P〈0.01）; the use of Naoyikang can significantly decrease the expression of p-tau （Ser404） and GSK-3β, whereas the expression of PP-2A was increased remarkably（P〈0.01）. Conclusion Naoyikang can attenuate tau hyperphosporylation of AD rats by activating the expression of PP-2A and inhibiting the expression of GSK-3β.

关 键 词：阿尔茨海默病 脑益康 TAU蛋白 蛋白磷酸酯酶2A 糖原合酶激酶-3Β 大鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]