人血清中抗禽戊型肝炎病毒抗体的检测与鉴定  

Detection and characterization of human serum antibodies against avian hepatitis E virus

作　　者：张红霞[1] 荆胜涛[2] 周恩民[2,3] 孙培明[2] 董施伟[2] 李艳菊[1] 

机构地区：[1]山东农业大学校医院,山东泰安271018 [2]山东农业大学动物科技学院,山东泰安271018 [3]山东省动物疫病防控重点实验室,山东泰安271018

出　　处：《中国卫生检验杂志》2009年第8期1741-1744,共4页Chinese Journal of Health Laboratory Technology

基　　金：家畜疫病病原生物学国家重点实验室开放课题基金;山东省"泰山学者"建设工程资助课题

摘　　要：目的:检测与鉴定人血清中抗禽戊型肝炎病毒(HEV)抗体。方法:应用禽HEV ORF2基因重组抗原(ORF2-1)和肽抗原(Pep-8和Pep-9)以间接ELISA和Western blot方法检测和鉴定7234份人血清中抗禽HEVORF IgG抗体。结果:从7234份人血清中检出66份(阳性率0.91%)抗禽HEV ORF2抗体ELISA阳性血清,进行Western blot方法验证为特异性抗禽HEV ORF2抗体,其中28份血清(阳性率42.4%)含有特异性抗禽HEV肽抗原Pep-8或Pep-9抗体,其中女性21人,男性7人,HBsAg阳性3人。结论:青年人群血清中存在特异性抗禽HEV抗体,阳性率为0.387%,女性抗禽HEV ORF2抗体阳性率为男性的3倍,说明青年女性比青年男性更有可能感染禽HEV。Objective： To detect and characterize human serum antibodies against avian hepatitis E virus（HEV）.Methods：Truncated recombinant avian HEV ORF2,designated ORF2-1,with a sequence between 339 and 570 of C terminal of ORF2 was produced in E coli.The synthetic peptides,named Pep-8 and Pepp-9,with aa sequences between 473 and 492 and between 477 and 492,respectively,were synthesized.ORF2-1 antigen was used in an indirect ELISA to screen 7234 human serum samples collected from young and healthy individuals.The ORF2-1 positive serum samples were first confirmed by the Western blot analysis and further characterized by direct binding to Pep-8 and Pep-9 antigen in an indirect ELISA.Results：From 7234 human serum samples screened against avian HEV ORF2-1 by the indirect ELISA,66 were detected positive and confirmed by the Western blot assay.The positive rate of human serum antibodies against avian HEV ORF2 was 0.91%.Further characterization of 66 ORF2-1 positive samples demonstrated that 19 samples were positive for Pep-8,17 positive for Pep-9 and 8 positive for both Pep-8 and Pep-9 with the specific anti-avian HEV antibody rate of 42.4%.These avian HEV ORF2 antibody positive human serum samples were from 21 female and 7 male young and healthy individuals with the ratio of female to male of 3：1.Conclusion：The results presented in this study demonstrated that sera from young and healthy individuals contained antibodies specific against avian HEV ORF2 antigen with the positive rate of 0.387%,suggesting that avian HEV could infect humans.The anti-avian HEV ORF2 positive rate of female and male was 3：1 indicating female has higher potential of being infected with avian HEV than male like that in human HEV infection.

关 键 词：戊型肝炎 禽戊型肝炎病毒 人抗禽戊型肝炎病毒抗体 血清学调查 

分 类 号：R-331[医药卫生]