肿瘤循环核酸的提取检测及临床应用  

Isolation, detection and clinical application of circulating nucleic acids in tumor

作　　者：高玉洁[1] 张伶[1] 

机构地区：[1]重庆医科大学医学检验系临床血液教研室临床检验诊断学省部共建教育部重点实验室,重庆400016

出　　处：《生命的化学》2009年第4期600-603,共4页Chemistry of Life

基　　金：国家自然科学基金项目(No.30872418)资助

摘　　要：循环核酸是一种细胞外游离状态的核酸,肿瘤患者血浆/血清中存在循环DNA、RNA和微RNA,具有片段小和含量低的特点。采用酚-氯仿抽提、硅胶或者磁珠载体分离法可提取微量的核酸片段,运用荧光分光光度法和PCR技术可观察循环核酸的改变。循环核酸检测可作为一种无创性的检测手段,应用于临床上肿瘤的早期诊断与鉴别、预后判断及疗效观察。Circulating nucleic acids in plasma or serum of cancer patients include circulating DNA, RNA and microRNA, which are characterized by small fragment and low content. Phenol-chloroform, silica gel and magnetic bead are often employed to extract the fragments of circulating nucleic acid, whose quantitative and qualitative changes were analyzed by fluorospectrophotometry and PCR respectively. Circulating nucleic acids are going to find their way of non-invasive methods in the early diagnosis aad differentiation, prognostic evaluation and monitoring of tumors.

关 键 词：循环核酸 血液 微小RNA 肿瘤 临床应用 

分 类 号：R730.4[医药卫生—肿瘤]