六种不同波长激光照射及光敏剂HpD对活体小鼠肿瘤表面漫反射率的影响  

The Effect of Six Different Laser Wavelengths and HpD on Diffuse Reflectance of Tumor Surface in Mice

作　　者：李晓原[1,2] 谭润初[1,2] 史宏敏[1,2] 魏华江[1,2] 

机构地区：[1]中山医科大学激光医学研究室 [2]广东药学院物理教研室

出　　处：《光电子．激光》1998年第4期341-343,共3页Journal of Optoelectronics·Laser

摘　　要：本文研究了激光波长及光敏剂对小鼠肿瘤表面的漫反射率的影响。实验采用一套积分球系统及三种激光器（Ａｒ＋激光器，Ｈｅ－Ｎｅ激光器，Ｎｄ：ＹＡＧ激光器）的六种波长的激光（４７６．５ｎｍ，４８８．０ｎｍ，４９６．５ｎｍ，５１４．５ｎｍ，６３２．８ｎｍ，１０６０ｎｍ），测定了注射光敏剂和没有注射光敏剂两组小鼠Ｓ１８０肉瘤表面的漫反射率，结果表明，注射光敏剂和没有注射光敏剂的小鼠肿瘤表面对六种激光的漫反射率无明显差异（Ｐ＞０．０５）；提示在激光光动力学疗法中，计算实际进入肿瘤组织的光剂量时，可以不考虑ＨｐＤ的影响。漫反射率随激光变化的曲线显示，在蓝光和红光范围内，肿瘤表面的漫反射率在５１４．５ｎｍ处有最小值，但总的来说，漫反射率的值变化不大；但在红外区的１０６０ｎｍ处，漫反射率值较大，提示在激光温热疗法中计算光剂量时，应考虑漫反射的影响。We report the effect of laser wavelengths and HpD on diffuse reflectance of tumor surface in mice.Three kinds of lasers (Ar +,Nd:YAG and He Ne lasers) of six different wavelengths and hemetoporphyrin derivative (HpD) as photosensitizer were used in the experiment.Tumor bearing mice were divided into two groups.The mice of one group were given an i.p.injection of HpD 10mg/kg.24 hours later,the laser diffuse reflectance of tumor surface of the mice of two groups were measured by a single integrating sphere system with laser in six wavelengths (476.5nm,488.0nm,496.5nm,514.5nm,632.8nm,and 1060nm).The results of measurement showed that significant difference of diffuse reflectance on tumor surface between the visible laser light and infrared laser light were observed.There was no significant difference in diffuse reflectance on tumor surface with and without i.p.injection of HpD.So it is unnecessary to consider the effect of HpD when calculating the actual laser dose irradiated into the tumor.The diffuse reflectance of tumor surface reaches its maximum at 1060nm wavelength,as twice as at 514.5nm wavelength.It is hence concluded that diffuse reflectance should be taken into consideration when calculating the actual laser density for irradiation on tumors.

关 键 词：血卟啉衍生物 小鼠 肿瘤 漫反射 激光疗法 

分 类 号：R730.57[医药卫生—肿瘤]