5-LOX和Caspase-1在大鼠脑缺血中的表达意义  

Expression of 5-LOX and Caspase-1 after Cerebral Ischemia in Rats

作　　者：杨卫忠[1] 涂献坤[1] 石松生[1] 陈春美[1] 王春华[1] 张永亮[1] 蓝佛琳[1] 

机构地区：[1]福建医科大学附属协和医院神经外科,福建省神经外科研究所,福州350001

出　　处：《脑与神经疾病杂志》2009年第2期118-120,共3页Journal of Brain and Nervous Diseases

摘　　要：目的研究大鼠脑缺血后5-脂氧合酶(5-lipoxygenase,5-LOX)和Caspase-1蛋白和基因的表达情况及其相关性。方法用线栓法制备大鼠脑缺血(MCAO)模型,用逆转录聚合酶链反应(RT-PCR)和免疫组化测定缺血脑组织6、12、24、48h不同时点脑皮质5-LOX和Caspase-1在基因和蛋白水平的表达。结果TTC脑片染色证实脑缺血模型建立成功,发现5-LOX和Caspase-1均在正常脑组织中少量表达,且随缺血时间延长表达量增高。结论大鼠脑缺血可诱导5-LOX和Caspase-1表达增高,二者可能参与缺血性脑损伤的形成。Objective To explore the expression of 5-1ipoxygenase（5-LOX） and Caspase-1 on both protein and gene levels after cerebral isehemia injury in rats. Methods The cerebral isehemia model was made by thread embolism of middle cerebral artery. The expression of 5-LOX and Caspase-1 were measured in cortex at different time points by the reverse-transcription PCR and immunohistochemistry. TTC staining was performed on brain sections. Results Expression of 5-LOX and Caspase-1 in ischemie cortex were increased along with the ischemic time. Con- elusion The cerebral ischemia leads to expression of 5-LOX and Caspase-1, and they might contribute to occur- rence of cerebral ischemia injury.

关 键 词：脑缺血 5-脂氧合酶 CASPASE-1 

分 类 号：R743.32[医药卫生—神经病学与精神病学]