改良阿霉素肾病大鼠模型的建立  

Establishment of a modified model of adriamycin nephrosis in rats

作　　者：杨维娜[1] 于琳华[2] 郭尚温[2] 成少利[3] 柴云[3] 

机构地区：[1]西安交通大学医学院人体解剖学与组织胚胎学系,陕西西安710061 [2]西安交通大学医学院病理学系,陕西西安710061 [3]西安交通大学医学院形态中心,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2009年第4期445-448,452,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨阿霉素肾病在不同病理发展阶段动物模型的制作。方法采用间隔2周两次尾静脉注射阿霉素的方法复制大鼠阿霉素肾病模型,观察各组大鼠血、尿生化指标和肾组织病理学的改变。结果该模型表现为严重水肿、大量蛋白尿、低蛋白血症和高脂血症;病理学检查显示,4周末肾小球足细胞肿胀,系膜细胞轻度增生;8周末有球囊粘连,肾小管上皮细胞萎缩;12周末部分肾小球节段性轻、中度硬化,间质内有明显灶性淋巴细胞浸润,并有轻度纤维化。结论经改良间隔2周两次尾静脉内注射阿霉素4mg/kg,可以成功复制阿霉素肾病模型。急性模型病理学改变类似人的微小病变性肾病,慢性模型病理学改变类似人的局灶节段性肾小球硬化。Objective To study the adriamycin nephrosis model in different development pathological stages. Methods The rat adriamycin nephrosis model was established by injecting adriamycin into tail-vein twice every two weeks to detect blood and urina biochemical indicators and to observe the pathological changes of the renal tissues. Results The model showed serious edema, proteinuria, hypoproteinemia, and hyperlipidemia. Podocytes were swollen and mesangial cells developed mild hyperplasia at the end of the fourth week. The nephric tubule atrophied at the end of the eighth week accompanied with adhesion between glomeruli and Bowman＇s capsule. Glomeruli sclerosis of mild or medium degree was observed at the end of the twelfth week with obvious lymphocyte infiltration in the renal interstitium as well as the formation of collagen fibers. Conclusion The adriamycin nephrosis model was successfully developed by injecting adriamycin 4 mg/kg into tail-vein twice every two weeks. The acute model is similar to human minimal change disease, and the chronic model is similar to human focal segmental glomerulosclerosis.

关 键 词：阿霉素肾病 足细胞 微小病变性肾病 局灶节段性.肾小球硬化 

分 类 号：R365.69[医药卫生—病理学] R181.2[医药卫生—基础医学]