止血潜能实验在血栓前状态(PTS)高危人群中的应用  

Application of overall hemostasis potential experiment in the high-risk population of prethrombotic state

作　　者：吴锋[1] 马西[1] 李晓会 车金[1] 

机构地区：[1]西安交通大学医学院遗传与分子生物学系,陕西西安710061 [2]第二附属医院影像中心,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2009年第4期515-517,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨总体止血潜能(OHP)实验在血栓前状态(PTS)高危人群中的应用价值。方法在尿激酶(UK)存在下,以低浓度组织因子(TF)激活血浆,在340nm处实时监测纤维蛋白形成与降解过程中的吸光度变化,从获得的凝血-纤溶图求出OHP、总体凝血潜能(OCP)和总体纤溶潜能(OFP)。应用该法检测包括恶性肿瘤(n=52)、冠心病(n=31)、妊娠中晚期孕妇(n=27)在内的PTS高危人群,并以100名正常人作为对照;同时检测PTS高危人群的血浆纤维蛋白原(FIB)含量,探讨其与OHP的相关性。结果恶性肿瘤、冠心病患者及中晚期妊娠妇女OCP和OHP值普遍增高(P<0.01),而OFP降低(P<0.01),表明此类人群明显存在PTS。恶性肿瘤、冠心病患者FIB含量增加,且与OHP的相关性良好(r=0.813、0.886,P<0.01),而妊娠妇女OHP的增高与FIB无明显相关性(r=0.298,P>0.05)。结论OHP检测方法能够反映机体止血平衡,可作为PTS高危人群的筛选方法。Objective To investgate the application of overall hemostasis potential （OHP） experiment in the high-risk population of prethrombotic state （PTS）. Methods The change of absorbance in fibrin formation and degradation was measured with a spectrophotometer at 340 nm when the plasma clotting was triggered by the low concentration of TF in the presence of urokinase. The OHP, overall coagulation potential （OCP） and the overall fibrinolysis potential （OFP） were obtained from the coagulation-fibrinolysis curve based on the computer analysis. To evaluate this OHP method, 52 cancer patients, 31 coronary artery disease patients, 27 mid/late-stage pregnancy women and 100 healthy controls were detected. In addition, the plasma fibrinogen was detected and its correlation with OHP was studied. Results The level of OCP and OHP in PTS high-risk population was significantly higher in cancer, coronary heart disease patients and the mid/late-stage pregnancy women than in the healthy controls （P 〈 0.01）, but the level of OFP was significantly lower （P 〈 0.01）. In addition, fibrinogen of cancer, coronary heart disease patients was significantly increased compared to that of the healthy controls （P 〈 0.05）, and had a good correlation with OHP （r = 0. 813, 0. 886, P 〈 0.01）. However, the level of OHP in pregnant women was unrelated to fibrinogen concentration （r = 0.298, P 〉 0.05）. Conclusion The OHP assay may indicate the hemostatic balance; therefore, it can be used for evaluation of PTS.

关 键 词：总体止血潜能 血栓前状态 纤维蛋白原 

分 类 号：R364[医药卫生—病理学]