星形胶质细胞在MPTP帕金森小鼠脑内的变化研究  

The Changes of Astrocytes in MPTP Parkinson's Mice

作　　者：邓广斐[1] 胡景鑫[1] 方亮[1] 

机构地区：[1]广州医学院生理学教研室,广东广州510182

出　　处：《现代生物医学进展》2009年第14期2622-2624,共3页Progress in Modern Biomedicine

摘　　要：目的:研究神经毒素1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methy-4-phenyl-1,2,3,6-tetrahy-dropyridine,MPTP)对小鼠脑内星形胶质细胞及肿瘤坏死因子-α(Tunlornecrosis factor alpha,TNF-α)的影响,了解MPTP致帕金森病发病机制。方法:将神经毒素MPTP注入C57BL/6小鼠腹腔内,制备帕金森病动物模型。观察注药后小鼠行为学变化,免疫组化检测各时间点多巴胺能神经元缺失和星形胶质细胞增生与激活情况,以及TNF-α表达水平的变化。结果:MPTP组黑质多巴胺(dopamine,DA)能神经元的数量随注射天数增加而持续减少,星形胶质细胞数量明显增高,GFAP及TNF-α在模型组黑质内有中强阳性表达,与对照组相比差异有统计学意义(P<0.05)。结论:MPTP可诱导星形胶质细胞的激活和增生,启动脑内炎症反应而介导DA神经元死亡。Objective： To investigate the local changes of astrocytes, tunlornecrosis factor alpha of different period in Parkinson＇s disease （PD） mouse model induced by 1-methy-4-phenyl-1, 2, 3, 6-tetrahy-dropyridine （MPTP）. Methods： Eight-week-old male C57BL/6 mice were divided into 2 groups randomly, and were given intraperitoneally at a dose of 30 mg/kg MPTP and saline （as control）, respectively. At the 1, 4, 7 days, the mice were sacrificed for immunohistochemistry and biodistribution studies. Results： Compared with the control group, there is a significant reduction of TH positive cells, while an increase of GFAP and TNF-α positive cells. Conclusions： The neurontoxin MPTP could induce the degeneration of DA neurons in the SN, activation and proliferation of astrocytes could lead to the releasing ofproinflammatory factors such as TNF-, which results in the apoptosis of DA neurons.

关 键 词：星形胶质细胞 帕金森病 TNF-Α 

分 类 号：Q95-3[生物学—动物学] R742.5[医药卫生—神经病学与精神病学]