p53基因药物抑制人鼻咽癌生长的免疫组化研究  

Researeh of gene therapy in human nasopharyngeal cancer with Adenovirus-mediated p53 by immunohistochemistry assay

作　　者：敖敏[1] 何刚[1] 

机构地区：[1]四川省人民医院耳鼻咽喉科,四川成都610072

出　　处：《四川医学》2009年第8期1193-1194,共2页Sichuan Medical Journal

摘　　要：目的探索p53基因在鼻咽癌基因治疗方面的可行性。方法以人鼻咽癌CNE细胞株为实验对象,将重组人p53腺病毒药物(1010rAd/p53)感染人鼻咽癌CNE细胞,用免疫组化实验的方法观察重组人p53腺病毒药物(rAd/p53)对人鼻咽癌CNE细胞体外生长的影响。结果重组人p53腺病毒药物(1010rAd/p53)对人鼻咽癌CNE细胞生长有明显抑制。结论重组人p53腺病毒药物(rAd/p53)对人鼻咽癌CNE细胞生长能有效抑制,为鼻咽癌的基因治疗提供了实验依据。Objective To explore the potential use of p53 in gene therapy for human nasopharyngeal cancer. Methods A human nasopharyngeal cancer cell line CNE was used. Adenoviral-mediated transfer of p53 gene（ rAd/p53 ）was transiently in- troduced into CNE cells in vitro. The growth of CNE cells in vitro was examined with immunohistochemistry assay. Results Cell growth was greatly suppressed in vitro. Conclusion rAd/p53 is a potential approach to the gene therapy of nasopharyngeal cancer.

关 键 词：鼻咽癌 CNE细胞 重组人P53 腺病毒 基因治疗 免疫组化 细胞培养 生长抑制 

分 类 号：R739.63[医药卫生—肿瘤]