重组人促红细胞生成素促进大鼠背部皮瓣成活及机制探讨  

Efficacy and mechanism of recombinant human erythropoietin for augmentation of viability on rats random flap

作　　者：李侃[1] 练慧斌[1] 徐利刚[1] 章宏伟[1] 

机构地区：[1]南京医科大学第一附属医院整形烧伤外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2009年第8期1108-1111,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技创新基金项目(CX2002008)

摘　　要：目的:观察重组人促红细胞生成素(rhEPO)对SD大鼠背部皮瓣的影响,探讨可能的机制。方法:以手术形成大鼠背部任意瓣(2cm×9cm)为模型,应用比色法和硫代巴比妥法观察术后24h组织中髓过氧化物(MPO)活性及丙二酰二醛(MDA)含量,评价rhEPO对MPO活性和MDA含量的影响;应用大体观察法,测定术后7天皮瓣存活率,评价rhEPO对皮瓣成活率的影响;光镜下观察CD34免疫组化染色切片,评价rhEPO对皮下微血管生成的影响。结果:rhEPO可明显降低术后24h时MPO活性和MDA含量(P<0.05),提高术后7天的皮瓣成活率(P<0.05),增加皮下微血管生成(P<0.05)。结论:rhEPO是通过减少中性粒细胞浸润,从而提高皮瓣存活率,其具体机制有待进一步研究。Objectlve：To observe the effects of recombinant human erythropoietin （rhEPO）on skin flap in the SD rats,and discuss the possible mechanism. Methods：A dorsal, caudally based random flap measuring 2 cm × 9 cm was created in all 24 SD rats which were divided into two groups：control group （Group A,n=12）with saline injected through the intraperitoneal route after operation,and treated group（Group B ,n=12）with single-dose rhEPO 5 000 IU/kg injected through the intraperitoneal route after operation. At 24 th hour postoperatively,the myeloperoxidase（MPO）activity and the amount of malondialdehyde（MDA） were evaluated on the flap tissue samples of 6 rats chosen randomly. The flap viability and immunohistochemical（CD34）methods which can evaluate the mierovaseular network were applyed on the rest rats at day 7 after operation. Results：There was significant decrease in MPO and MDA in treated group compared with control group,and a significant increase in the flap viability in treated group was observed（P 〈 0.05）. Conclusion： rhEPO can decrease the MPO activity and amount of MDA in the tissues of random flap at the first 24 h after operation and increase mierovessel density and viability at day 7 after operation.

关 键 词：重组人促红细胞生成素 任意皮瓣 SD大鼠 

分 类 号：R364.2[医药卫生—病理学]