野生型P53和C-Ha-ras基因在顺铂诱导膀胱癌细胞凋亡中的表达及意义  

The expression of wildtype P53 and CHaras in bladder cancer cells with apoptosis induced by cisplatin (CDDP)

作　　者：张廷继[1] 陈一戎[1] 王志平[1] 段国兰[1] 刘国栋[1] 徐晨阳[1] 史庭恺[1] 

机构地区：[1]兰州医学院第二附属医院泌尿外科研究所

出　　处：《中华泌尿外科杂志》1998年第7期397-400,共4页Chinese Journal of Urology

摘　　要：应用荧光原位杂交技术观察顺铂诱导膀胱癌细胞株ＢＩＵ８７体外细胞凋亡过程中野生型Ｐ５３和ＣＨａｒａｓ基因的表达，探讨抗癌药物诱导膀胱癌细胞凋亡过程中抗癌基因和癌基因的作用。结果显示：顺铂能够明显地诱导膀胱癌细胞凋亡，具有一定范围内的作用时间和药物浓度的依赖性。随凋亡细胞数上升，野生型Ｐ５３表达增强，而ＣＨａｒａｓ基因表达下降，提示膀胱癌细胞凋亡可能是顺铂的杀瘤效应途径之一。Ｐ５３和ＣＨａｒａｓ基因均参与了细胞凋亡的调控过程，野生型Ｐ５３基因可能是通过下调ＣＨａｒａｓ基因的表达而发挥其抑制细胞增殖活性，促进凋亡的发生。The expression of P53 and CHaras of BIU87 bladder cancer cell lines with apoptosis induced by CDDP was detected by means of fluorescence immuno in situ hybridization (FISH).An extensive apoptosis in BIU87 cells treated by CDDP has occured, which was dependent on an apropriate concentration of the drug or treatment time. The positive expression rate of P53 has been significantly upregulated and CHaras was downregulated as apoptotic index increased. These results suggested enhanced expression of P53 or decreased expression of CHaras might be involved in apoptosis or inhibition of the proliferation of bladder cancer cells induced by CDDP.

关 键 词：膀胱肿瘤 基因 细胞凋亡 顺铂 药物疗法 

分 类 号：R737.140.5[医药卫生—肿瘤]