穿龙薯蓣皂甙酸解液中鼠李糖的提取  

Extraction of Rhamnose from Acidolysis Liquor of Dioscorea Nipponica Makino Dioscin

作　　者：肖芳[1] 郑小江[2] 谭远友[1] 赵宗梁[1] 陈祖玉[1] 刘传凤[1] 陈英明[1] 赵丽荣[1] 

机构地区：[1]武汉科技学院环境与城建学院,湖北武汉430073 [2]湖北民族学院生物科学与技术学院,湖北恩施445000

出　　处：《化学与生物工程》2009年第8期54-57,共4页Chemistry & Bioengineering

基　　金：湖北省生物资源与保护重点实验室资助项目(2007004)

摘　　要：从穿龙薯蓣酸解液中成功提取了薯蓣皂甙。鲜穿龙薯蓣经磨碎、过筛、离心、沉淀、醇提,除去纤维素、水溶物、淀粉,得到总皂甙;再经酸解、过滤、酵母菌发酵消耗葡萄糖,最终得到鼠李糖。确定鼠李糖的最佳提取工艺为:100 g鲜穿龙薯蓣醇提3次,每次醇用量为40 mL;0.14 MPa下,用120 mL 0.5 mol.L-1的H2SO4水解1 h;酵母菌发酵时无需添加营养物质,发酵时间不短于17 h。此工艺下鼠李糖得率为0.196%。采用薄层层析确认产品为鼠李糖,用DNS法测定发酵液中鼠李糖含量,具有准确性和易操作性。Rhamnose extraction technology from acidolysis liquor of Dioscorea nipponica Makino dioscin was studied. The experiment indicated that the treatment of grinding, filtrating, centrifuging, precipitating and alcohol-extracting was available to remove fiber, water-soluble matter, starch and get total dioscin; after the process of hydrolysis, fitrating, neutralization and eliminating the glucose in the filtration liquid with yeast, rhamnose was obtained. The optimum conditions for the extraction of rhamnose were as follows： 100 g Dioscorea nipponica Makino was alcohol-extracted for 3 times with 40 mL per time;acidolyzed with 120 mL 0. 5 mol · L^-1 H2SO4 at 0. 14 MPa for 1 h,and fermentation time was not less than 17 h without nutrition. In this way,the rhamnose yield was 0. 196%. DNS method was accurate and convenient to measure the content of rhamnose in the fermentation liquid. The TLC analysis indicated that the product obtained was rhamnose.

关 键 词：穿龙薯蓣 薯蓣皂甙 鼠李糖 提取 

分 类 号：TQ028[化学工程]