结核分枝杆菌单克隆抗体与自身抗原组蛋自的免疫交叉反应研究  

Study on Immune Cross-reaction of Mycobacterium Tuberculosis MAb with Self-antigen Histone

作　　者：李琰[1] 陈佩丽[1] 刘文[1] 胡巍 王洪海[1] 庄玉辉[3] 刘为平[1] 朱琪泉 

机构地区：[1]复旦大学 [2]山东淄博师专 [3]北京解放军309医院

出　　处：《复旦学报（自然科学版）》1998年第4期419-422,共4页Journal of Fudan University：Natural Science

基　　金：复旦大学遗传工程国家重点实验室资助

摘　　要：WesternBlot实验证实了结核分枝杆菌（Mycobacteriumtuberculosis）H37Ra的单克隆抗体与自身抗原组蛋白发生免疫交叉反应．利用计算机软件对交叉反应的分子生物学基础进行了分析，发现结核分枝杆菌H37Ra的蛋白与组蛋白H1，H2A，H2B，H3，H4各有一个包含3个或4个氨基酸的序列同源区域．Cross-reaction of Mycobacterium tuberculosis H37Ra monoclonal antibody (MAb)with self-antigen histone were demonstrated by western Blotting analysis. The molecular basis of the cross-reaction was investigated by amino acid sequence analysis using computer software. Homologous sequence of three or four amino acids between H37Ra protein and histone were found.

关 键 词：结核分枝杆菌 组蛋白 免疫交叉反应 单克隆抗体 

分 类 号：R378.911[医药卫生—病原生物学] R392.11[医药卫生—基础医学]