生物制品中支原体污染的快速检测  

Fast Testing of Biological Products for Mycoplasma Contamination

作　　者：应蓓蓓[1] 陆许华 关维寿 赵寿元[1] 李昌本[1] 

机构地区：[1]复旦大学 [2]上海市南市区卫生防疫站

出　　处：《复旦学报（自然科学版）》1998年第4期491-495,共5页Journal of Fudan University：Natural Science

基　　金：上海市自然科学基金

摘　　要：用两步PCR法（NestedPCR）及DNA荧光染色技术（HoechstStaining），检测了国内部分生物制品研究所生产的81份不同来源和批号的疫苗及一些实验用细胞株中的支原体，并对检测结果做了比较．PCR阳性检出率为38．3％，Hoechst阳性检出率为19％．这些结果表明PCR法较Hoechst染色法更敏感，同时亦提示必须重视检测与控制国内生物制品的支原体污染问题．Tests of the biological products for mycoplasma contamination have since been an important step of quality control. 81 different samples including virus vaccines and cell lines were tested using a fast and sensitive nested PCR. The results showed that 31 samples were contaminated by different kinds of mycoplasma. However, only 15 out of these 31 samples were positive with Hoechst staining method. These data suggest that nested PCR method for mycoplasma detection could be a potential tool for standard quality control of biological products.

关 键 词：支原体污染 DNA荧光染色法 生物制品 两步PCR法 

分 类 号：R392－33[医药卫生—免疫学] R375[医药卫生—基础医学]