含硒桥联环糊精诱导体外培养H22小鼠肝癌细胞凋亡的作用  

Apoptosis of mouse H22 cell cultured in vitro induced with 2-selenium bridged β-cyclodextrin

作　　者：许新[1] 王冰梅[1,2] 王微[1] 潘宇[2] 林婷婷[2] 闫岗林[2] 罗贵民[2] 

机构地区：[1]长春中医药大学研发中心,吉林长春130117 [2]吉林大学分子酶学工程教育部重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第4期604-607,F0002,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(20572035)

摘　　要：目的:研究含硒桥联环糊精(2-SeCD)对体外培养H22小鼠肝癌细胞凋亡的促进作用,探讨2-SeCD的抗肿瘤活性。方法:体外培养的H22细胞分别加入80、160、320和640μmol.L-12-SeCD作为实验组,并设阴性对照组。倒置相差显微镜观察细胞形态的变化;MTT法检测细胞生长抑制率,计算中效抑制浓度(IC50);流式细胞术(FCM)检测细胞周期和凋亡。结果:320和640μmol.L-12-SeCD组细胞生长抑制率分别为(25.90±2.13)%和(49.40±3.66)%,与对照组比较差异有显著性(P<0.01),2-SeCD 48 h的IC50为611.29μmol.L-1;320和640μmol.L-12-SeCD组凋亡率分别为5.52%和12.47%,2-SeCD可使细胞阻滞于G0/G1期。结论:2-SeCD可抑制H22细胞增殖并促进其凋亡,2-SeCD对肝癌具有潜在的治疗价值。Objective To study the effects of 2-selenium bridged β-cyclodextrin（2-SeCD） on the apoptosis of H22 cell line and explore its activity of anti-tumor.Methods 2-SeCD with different doses （80,160,320,640 μmol·L^-1 were added into the medium in which the cells were cultivated in vitro, negative control group was set up also.The morphological changes of H22 cells were observed under phase contrast microscope;the inhibibory rate of the growth of H22 cells was detected with MTT method,IC50 was calculated,the cell cycle and apoptosis of H22 cells were determined with flow cytometry.Results After exposure of H22 cells to 2-SeCD at the doses of 320 and 640 μmol·L^-1or 48 h,the inhibitory rates of H22 cells were（25.90±2.13）% and（49.40±3.66）%,they were higher than that in control group（P〈0.01）.2-SeCD inhibited the growth of H22 cells in dose-dependent manner,and its IC50 was 611.29 μmol·L^-1After exposure of H22 cells to 2-SeCD at the doses of 320 and 640 μmol·L^-1for 48 h,the apoptotic rates were 5.52% and 12.47%,respectively. 2-SeCD inhibited the proliferation of cell cycle at G0/G1 phase.Conclusion 2-SeCD can suppress the growth of H22 cells and induce apoptosis,and it has a potential treatment value in inhibiting hepatoma cell proliferation.

关 键 词：含硒桥联环糊精 H22小鼠肝癌细胞 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]