胶原膜复合rhBMP-2引导牙周组织再生的组织学观察  

Histological observation of periodontal tissue regeneration guided with collagen membrane combined with recombinant human bone morphogenetic protein-2

作　　者：陶安军[1] 林崇韬[1] 于海霞[1] 李红艳[1] 申玉芹[1] 

机构地区：[1]吉林大学口腔医院牙周病科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2009年第4期673-676,766,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(20020658)

摘　　要：目的:探讨胶原膜复合rhBMP-2对大鼠牙周组织缺损模型牙周组织再生修复的影响。方法:18只健康雄性Wistar大鼠,于下前牙龈颊沟冠方1 mm处制备实验性牙周组织缺损模型,随机分为对照组、胶原膜组(Co组)和胶原膜复合rhBMP-2组(Co/rhBMP-2组)。术后4、6和8周分批处死大鼠,取实验部位进行组织学观察。结果:Co/rhBMP-2组术后4周缺损区可见大量新生骨;6周新生骨充满缺损区;8周新生牙槽骨密度较高,根面有新生牙周膜和牙骨质样组织,未见牙龈上皮长入。Co组术后4周缺损边缘可见新骨形成;6周新生骨量增多,但尚未充满缺损区;8周根面有少量牙周膜和牙骨质样组织形成。对照组术后4周缺损边缘有少量新骨沉积;6周缺损边缘可见束状胶原纤维;8周切迹内仅有少量新生牙周组织形成,并有牙龈上皮长入。结论:胶原膜复合rhBMP-2既有引导作用,防止牙龈上皮长入,维持生长空间;又具有诱导骨形成作用,能促进牙周组织修复再生。Objective To explore the effect of collagen membrane combined with recombinant human bone morphogenetic protein-2（rhBMP-2） on periodontal tissue regeneration and repair in periodontal defect models of rats.Methods Eighteen male healthy Wistar rats,which were made experimental periodontal bone defects in the inferior incisors,were randomly divided into three groups：control group,collagen membrane group（Co）,collagen membrane and rhBMP-2 group（Co/rhBMP-2）.Two rats from each group were sacrificed at 4,6,8 weeks after operation.The mandibles were removed,and examined under light microscope.Results In Co/rhBMP-2 group： 4 weeks after operation,a large amount of bone formed in the defects;6 weeks later,new bone filled in the defects;8 weeks later,alveolar bone emerged,newly-formed periodontal ligament and cementum localized at the root edges,no gingival epithelium was observed.In Co group：4 weeks after operation,new bone formed at the edge of the defects;6 weeks later,a larger amount of bone and periost were observed;8 weeks later,a small amount of periodontal ligament and cementum localized at the root edges.In control group： 4 weeks after operation,there was a small amount of newly-formed bone at the edge of the defects;6 weeks later,collagen fibers were found at the edge of defects;8 weeks later,a small amount of periodontal tissue reached its original height and gingival epithelium proliferated obviously.Conclusion Collagen membrane combined with rhBMP-2 which has not only conductive effect but also effect of osteoinduction,can prevent the long-epithelium growing,maintain the growth gap,and promote periodontal tissue to regenerate.

关 键 词：胶原膜 重组人骨形成蛋白2 引导组织再生 

分 类 号：R781.4[医药卫生—口腔医学]