抑癌基因PTEN单核苷酸多态性与胃癌的关联性分析  

Analysis of association between PTEN gene single nucleotide polymorphism and stomach neoplasms

作　　者：宋朝霞[1] 刘冰[1] 赵佳[1] 刘军[2] 

机构地区：[1]吉林大学基础医学院医学遗传学教研室,吉林长春130021 [2]吉林大学第一医院肝胆胰外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第4期694-697,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200505187);长春市科技计划项目资助课题(2006044)

摘　　要：目的:探讨抑癌基因PTEN的两个单核苷酸多态性(SNP)位点rs2735343和rs701848与中国北方汉族人群胃癌的关系,阐明二者是否是胃癌发生的易感位点。方法:应用PCR-RFLP法检测58例胃癌患者(病例组)与104例健康者(对照组)的PTEN的SNP位点rs2735343和rs701848的基因型,应用SPSS 10.0分析PTEN的基因多态性与胃癌的关联性。结果:rs2735343位点的PCR扩增目的片段长度为272 bp,经酶切后呈现GG、GC、CC 3种基因型;rs701848位点的PCR扩增目的片段长度为394 bp,经酶切后呈现TT、TC、CC3种基因型;rs2735343与rs701848两个SNPs基因型分布在病例组(P=0.062;P=0.055)和对照组(P=0.246;P=0.692)均符合Hardy-Weinberg平衡;这两个SNPs在病例组的等位基因及基因型频数分布与对照组比较差异均无显著性(P>0.05)。结论:PTEN的SNP位点rs2735343和rs701848与中国北方汉族人群胃癌无关联性,可能不是此人群胃癌的易感位点。Objective To investigate the genetic assaciation between tow single nucleotide polymorphisms（SNPs）（rs2735343 and rs701848） of PTEN anti-oncogene and gastric carcinoma in Northern Han Chinese and to elucidate whether the two SNPs are susceptibility loci for gastric carcinoma in Northern Han Chinese.Methods The PCR-based restriction fragment length polymorphism（PCR-RFLP） analysis was used to detect these two SNPs present in the PTEN gene among 58 sporic gastric carcinoma patients（case group） and 104 normal individuals（control group）.The association between the polymorphisms and gastric carcinoma was analyzed with SPSS 10.0.Results The fragment of rs2735343 was 272 bp,and three genotypes（GG,GC,CC） were found after digestion;The fragment of rs701848 was 394 bp,and three genotypes（TT,TC,CC） were also found after digestion;The genotypic frequencies of rs2735343 and rs701848 were not deviated from Hardy-Weinberg equilibrium either in case group（P=0.062;P=0.055） or control group（P=0.246;P=0.692）.The allelic and genotypic frequencies of rs2735343 and rs701848 had no remarkable difference between case and control groups（P〉0.05）.Conclusion Neither rs2735343 nor rs701848 of the PTEN gene is not associated with gastric carcinoma,they may not be susceptibility loci for gastric carcinoma in Northern Han Chinese.

关 键 词：PTEN 单核苷酸多态性 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]