弥散加权成像评价内皮抑素对小鼠结肠癌疗效及其与肿瘤细胞密度的相关性研究  

Correlative Study of Diffusion-Weighted Imaging and Cellular Density in the Evaluation of Therapeutic Effect of Endostatin for Colorectal Carcinoma in Transplantation Tumor of Mice

作　　者：韩明[1] 程鑫[1] 张仕状[1] 王滨[1] 曲林涛[2] 

机构地区：[1]潍坊医学院医学影像学系,山东潍坊261042 [2]潍坊医学院附属莱阳中心医院

出　　处：《实用放射学杂志》2009年第8期1191-1194,共4页Journal of Practical Radiology

基　　金：国家自然科学基金资助项目（30572323,30772716）;潍坊医学院青年教师科研启动基金资助项目（KQ07002）.

摘　　要：目的探讨磁共振弥散加权成像（diffusion-weighted imaging，DWI）在评价内皮抑素对小鼠结肠癌疗效的应用价值及表观弥散系数（apparent diffusion coefficient，ADC）与肿瘤细胞密度的相关性。方法建立小鼠结肠癌皮下移植瘤模型，1周后筛选成瘤均匀的16只随机分成内皮抑素（ES）组和生理盐水（NS）组，分别给予内皮抑素和0．9％生理盐水各0．2ml，14d后行MRI检查，测量肿瘤的ADG值，并分析其与病理特征及肿瘤细胞密度的相关性。结果b=800s／mm^2时DWI图像质量可，肿瘤显示清晰；在b=800s／mm^2的ADC图像上，NS组与ES组的ADC值分别为0．87±0．21和1．64±0．34，二者间存在统计学差异（t=5．412，P〈0．001）；ES组肿瘤内部坏死较多，细胞密度较低（P〈O．01）。肿瘤ADC值与细胞密度呈明显负相关（r=-0．935，P〈O．001）。结论DWI能显示肿瘤内部微观结构，在内皮抑素对小鼠结肠癌疗效评价中具有重要价值。Objective To investigate the correlation between diffusion-weighted imaging and cellular density in the evaluation of therapeutic effect of endostatin for colorectal carcinoma in transplantation tumor models of mice. Methods 24 subcutaneously trans- plantation tumor models of eolorectal carcinoma （CT-26） in mice were established, one week later 16 tumor-bearing mice were sieved out and divided randomly into endostain（ES） group and normal saline （NS} group, treated with intraperitoneal injection of endostatin and equal volume of saline respectively for 14 days. Subsequently, MR1 examinations were performed, and ADC values of tumors were measured and compared with that of pathological characters and cellular density. Results The tumors could be seen by DWI at b=800 s/mm^2. The ADC values of NS group （0.87±0.21） were lower than that of ES group （1.64±0.34）, there＇was sig nificant difference （t= 5. 412,P〈0. 001）. Necrosis was more in ES group than that in NS group, and cellular density was lower in ES group than that in NS group （ P〈0.01 ） . ADC values were correlated negatively with the cellular density （ r =-0. 935 , P〈0. 001）. Conclusion DWI can observe the changes of molecular microstructure intratumorally after chemotherapy. The ADC values played an important role in evaluating the therapeutic effect of endostatin for colorectal carcinoma in transplantation tumor models of mice.

关 键 词：结肠癌 磁共振成像 内皮抑素 鼠 肿瘤瘤模型 

分 类 号：R735.35[医药卫生—肿瘤] R445.2[医药卫生—临床医学]