气升式反应器培养集胞藻6803过程中光能利用特性研究  

Studies on characteristics of light utilization during the culture of Synechocystis sp. PCC 6803 in airlift photobioreactor

作　　者：张志斌[1] 颜日明[1] 曾庆桂[1] 朱笃[1] 

机构地区：[1]江西师范大学生命科学学院,江西南昌330022

出　　处：《海洋通报》2009年第4期54-61,共8页Marine Science Bulletin

基　　金：国家自然科学基金(20506009);江西省自然基金(0530095);教育厅(GJJ08147)项目资助

摘　　要：在气升式光生物反应器中,对集胞藻6803分批培养条件下的光衰减、平均光强、光的分布以及藻细胞生长对光能的利用特性进行分析。结果表明:Lambert-Beer定律可较好描述细胞浓度及光程对光衰减的影响;随着藻细胞密度的增加,光生物反应器内平均光强不断减小,藻细胞的比光能吸收速率和比生长速率也不断降低,反应器中"暗区"体积在培养6d后超过总体积的80%;利用Pirt细胞生长生物能量模型描述比生长速率和比光能吸收速率的关系,在入射光强为58.73KJ/(m2·h),求得基于光能的细胞得率YG和维持系数m分别为9.98×10-3g/kJ和0.445kJ/(g·h)。The characteristics of light attenuation, average irradiance, light distribution and light utilization in air-lift photobioreactor during batch culture of Synechocystis sp. PCC 6803 were investigated. It was found that the adapted Lambert-Beer law could well simulate influences of cell concentration and optical length on light attenuation. While algal cell concentration increased, the average irradiance in the photobioreactor, the specific light energy absorbance rate and the specific growth rate decreased continuously, and the dark zone exceeded 80% of the volume of the photobioreactor after 6 day cultivation. The relationship between the specific growth rate and the specific light energy absorption was described by means of the Pirt＇s bioenergetic model of algal growth. The biomass yield and maintenance coefficients ofSynechocystis sp. PCC 6803 based on light energy were determined at 58.73KJ/（m^2.h） and amounted to 9.98× 10^-3g/kJ and 0.445kJ/（g-h） respectively.

关 键 词：集胞藻6803 光衰减 光分布 平均光强 光能利用 

分 类 号：Q947.8[生物学—植物学]