结肠癌SW480细胞FAP-1表达与奥沙利铂化疗耐药关系的研究  

Study on the relationship between the expression of FAP-1 of colon carcinoma cells and oxaliplatin chemo-resistance

作　　者：肖治宇[1] 李祖勇 吴畏[1] 伍刚[1] 姚和瑞[3] 

机构地区：[1]中山大学孙逸仙纪念医院外科,510120 [2]中山大学孙逸仙医院急诊科 [3]中山大学孙逸仙纪念医院肿瘤科

出　　处：《岭南现代临床外科》2009年第4期270-272,共3页Lingnan Modern Clinics in Surgery

基　　金：广东省自然科学基金资助项目(7001592);广东省科技计划资助项目(2006B36002010);广东省医学科研基金资助项目(A2005226)

摘　　要：目的探讨结肠癌FAP-1表达与其对奥沙利铂化疗耐药间的关系。方法用奥沙利铂处理结肠癌SW480细胞,用MTT法评价其细胞增殖能力,以RT-PCR法评价FAP-1 mRNA表达水平。结果奥沙利铂化疗仅能一定程度上抑制结肠癌SW480的增殖,但随作用时间的延长,SW480细胞仍能继续增殖。经过奥沙利铂化疗FAP-1的表达上调。结论奥沙利铂化疗可引起FAP-1表达的上调,增强肿瘤细胞对抗Fas诱导的细胞凋亡。这可能与结肠癌对奥沙利铂化疗的耐药相关。Objective To investigate the relationship between FAP-1 expression and the chemo-resistance to Oxaliplatin of human colorectal cancer ceil line SW480. Methods Mter Oxaliplatin administration, Methyl thiazolyl tetrazolium （MTT） assay was used to measure proliferation of SW480 ceils, RT-PCR was used to measure the FAP-1 mRNA lever. Results After oxaliplatin treatment, the proliferation of SW480 ceils could be reduced to a low degree, but as time went by, the SW480 cell could still proliferate. Oxaliplatin can up-grate FAP-1 expression in SW480. Conclusion Oxaliplatin could enhance the expression of FAP-1 of SW480 cells, thus, enhance the anti-Fas induced apoptosis of the tumor cells. This may account for the chemo-resistance to Oxaliplatin of colorectal cancer.

关 键 词：Fas相关磷酸酶-1 结肠癌 奥沙利铂 化疗耐药 

分 类 号：R735.3[医药卫生—肿瘤]