同一个体食管鳞癌和贲门腺癌中人乳头状瘤病毒感染和p53、p16蛋白改变的意义  

Detection and Significance of HPV16 Infection,p53 and p16 Expression in Concurrent Esophagus and Gastric Cardia Cancers from Same Patients at High-incidence Area in Henan

作　　者：丁广成[1,2] 董忠生[3] 王立东[1] 李吉林 郭军辉[1] 郭涛[1] 

机构地区：[1]河南省食管癌重点开放实验室,郑州大学第一附属医院,郑州大学基础医学院,河南郑州450052 [2]郑州大学第五附属医院肿瘤放射治疗科,河南郑州450052 [3]郑州铁路职业技术学院医学技术系,河南郑州450052 [4]河南省林州市姚村食管癌医院病理科,河南林州456592

出　　处：《中国临床医学》2009年第4期536-539,共4页Chinese Journal of Clinical Medicine

基　　金：河南省自然科学基金(0511043200);河南省医学科技重大攻关项目(WKJ2007-2-028)

摘　　要：目的:探讨同一个体食管、贲门双源癌的人乳头状瘤病毒(HPV)感染和p53、p16蛋白表达与食管和贲门癌变的关系。方法:采用聚合酶链式反应(PCR)检测17例双源癌食管鳞癌组织和贲门腺癌组织人乳头状瘤病毒16型(HPV16)感染特征,采用免疫组织化学方法检测p53和p16蛋白在17例双源癌鳞癌组织和腺癌组织中的表达。结果:17例双源癌组织中8例食管鳞癌组织,5例贲门腺癌组织中检测到HPV16-DNA(47%对29%),且有2例患者食管鳞癌和贲门腺癌组织中同时检测到HPV16-DNA的存在。p53和p16蛋白在两种组织中均有较高表达。p53蛋白突变率在HPV阳性组织中均较HPV阴性组织低(50%对77%;60%对66%);在HPV相关的双源癌中,p16蛋白表达在腺癌组织中较鳞癌组织高(75%对25%)。结论:同一个体食管鳞癌和贲门腺癌均有不同程度HPV感染,提示HPV可能是两者共同相关致病危险因素,高危型HPV16感染可能在食管鳞癌和贲门腺癌发生中起重要作用。p16蛋白表达在腺癌组织中较鳞癌组织高,有可能成为HPV相关性贲门腺癌的一个筛查指标。Objective： To investigate the correlations of HPV16 infection and p53, p16 expression in concurrent esophageal and gastric cardia carcinogenesis from same patients at high-incidence area in Henan. Methods： HPM16-DNA was deteded by polymerase chain reaetion（PCR）,expressions of p53 and p16 were detected by ABC immunohistochemistry in 17 surgically reseeted specimens from the concurrent esophageal and gastric cardia cancers（CC）. Results： The positive rate of HPV16E6-DNA was 47% （8/17） in esophageal squamous cell careinoma（ESCC）, 29% （5/17） in gastric eardia adenoeareinoma（GCA）, respectively. And HPV16E6- DNA was observed simultaneously in 2 ESCC and 2 GCA. The expression of p53 and p16 protein were observed both in ESCC and GCA tissues with different degrees. The expression rates for p53 in HPV positive and negative tissues were 50%,77% in ESCC, and 60%,66% in GCA, respectively. The expression of p16 protein was significantly higher in GCA than that in ESCC（75 % vs 25 %, P〉0. 05）. Conclusion：The consistent HPV infection in CC from the patients at same area suggest that HPV may be one of the common oncogenic factor for both ESCC and GCA. HPV16, the high-risk type, may play an important role in the development of ESCC and GCA. p16 may be another candidate biomarker for screening of HPV-related GCA.

关 键 词：食管鳞癌 贲门腺癌 人乳头状瘤病毒 聚合酶链式反应 免疫组化 

分 类 号：R735.1[医药卫生—肿瘤]