三氧化二砷对离体人胆囊癌细胞的放射增敏作用  

Enhancement of Radiation Response by Arsenic Trioxide on Human Gallbladder Cancer Cells in vitro

作　　者：潘洪涛[1] 艾志龙[1] 刘厚宝[1] 锁涛[1] 童赛雄[1] 

机构地区：[1]复旦大学附属中山医院普外科,上海200032

出　　处：《中国临床医学》2009年第4期540-541,共2页Chinese Journal of Clinical Medicine

基　　金：上海市科技发展基金(20044119702)

摘　　要：目的:观察三氧化二砷(As2O3)对人胆囊癌GBC-SD细胞的放射增敏作用。方法:0.2μmmol·L-1As2O3处理胆囊癌细胞后给予递增剂量(0、2、4、6、8、10Gy)的137Csγ射线照射,观察GBC细胞的存活率。结果:细胞存活率随放射剂量的增加逐渐下降;同等放射剂量下,As2O3处理的细胞存活率明显低于单纯照射组,Dq值明显下降,放射增敏比为1.5131。结论:As2O3对离体人胆囊癌细胞有放射增敏作用。Objective： To observe the enhancement effect of arsenic trioxide （As2O3 ） on response of human gallbladder cancer ceil to radiation. Methods： Cells were pre-treated with 0.2μmmol/L As2O3 and radiated with increasing doses of 137Cs T-radiation（0,2,4 ,6 ,8 and 10Gy,respectively）. The s urvivai fraction of cells were calculated. Results： Ceil survival rate decreased accompanied with the increased dose of radiation. As treated with the same dose radiation, the cell survival rate was lower in the combination of As2O2 and radiation than that of radiation alone , and the Dq value was decreased significantly. The radiation enhancement ratio was 1. 5131. Conclusion： As2O3 could enhance the radiation response of human gallbladder carcinoma ceils in vitro.

关 键 词：三氧化二砷 胆囊癌细胞 放射敏感性 

分 类 号：R735.8[医药卫生—肿瘤]