裸鼠前列腺癌PC-3M细胞移植瘤模型的建立及其Her-2/neu蛋白免疫组化检测  

Establishment of tumor xenograft model of PC-3M prostate cancer in nude mice and detection of Her-2/neu by immunohistochemistry

作　　者：章宜芬[1] 柏涛[1] 吴鸿雁[1] 戴玉田[2] 孙则禹[2] 

机构地区：[1]南京大学医学院附属鼓楼医院病理科,江苏南京210008 [2]南京大学医学院附属鼓楼医院泌尿外科

出　　处：《徐州医学院学报》2009年第8期512-515,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省"135工程"医学重点学科基金;2006年度人事部留学回国人员科技活动项目择优资助经费启动类项目;2006年度南京市医学科技发展项目计划(YKK06087)

摘　　要：目的观察人前列腺癌细胞株PC-3M中Her-2/neu蛋白表达情况。建立人前列腺癌PC-3M细胞裸鼠移植瘤模型,观察肿瘤组织形态并检测肿瘤细胞中Her-2/neu蛋白的表达。方法培养PC-3M细胞,制作细胞蜡块。将前列腺癌PC-3M细胞接种于裸鼠颈背部皮下,成瘤后切除肿块作石蜡切片苏木精-伊红染色观察肿瘤形态,肿块及PC-3M细胞蜡块均行免疫组化染色检测Her-2/neu蛋白的表达。结果细胞蜡块免疫组化示PC-3M细胞表达Her-2/neu蛋白。裸鼠接种PC-3M细胞后成瘤率达100%,苏木精-伊红染色检查示分化差的恶性肿瘤形态,免疫组化染色示肿瘤细胞表达Her-2/neu蛋白。结论人前列腺癌PC-3M细胞接种裸鼠成瘤率高,移植瘤模型保留了肿瘤的生物学特性及表达Her-2/neu蛋白免疫表型,为研究Her-2/neu蛋白在前列腺癌中的作用提供了较好的动物模型。Objective To investigate the expression of Her - 2/neu protein in human prostate cancer cell line PC - 3M, establish its animal model of tumor xenografts in nude mice, observe the histopathological features and detect the ex- pression of Her-2/hen protein. Methods PC -3M cells were cultured to make cell blocks for immunohistoehemical staining of Her- 2/neu protein. Prostate cancer cell line PC -3M cells were subcutaneously implanted under the nape of nude mice. The paraffin slices of the removed tumor from xenografts were examined by hematoxylin - eosin staining for morphological features. The tumor slices and PC -3M cell blocks underwent immunohistochemical staining for the detec- tion of Her - 2/neu protein. Results PC - 3M cells were immunopositive with Her - 2/neu protein on the cell blocks. The tumor- formation rate was 100% in the nude mice after inoculation with PC -3M cells. Morphologically, the tumor cells showed the morphological features of poorly differentiated malignancy. Immunohistochemical staining revealed that the tumor cells were also ilnmunopositive with Her - 2/neu protein. Conclusion The tumor - formation rate is high by subcutaneous xenografis of PC -3M cells in nude mice. The tumor xenograft model retains the biological features of PC - 3M cells and expresses Her- 2/neu protein, which suggests that it might be a good animal model for the study of Her- 2/neu protein in prostate cancer in vivo.

关 键 词：前列腺肿瘤 动物模型 裸鼠 HER-2/NEU蛋白 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]