动态磁场下微生物的生物学效应研究  

The Effect of Dynamic Magnetic Field on Microorganisms

作　　者：尹焕才[1,2] 薛小平[1] 杨慧[1] 唐蕊华[1] 谢玉为[1] 苏婧[1] 宋凯[1] 呼延霆[1] 汪桦[1] 

机构地区：[1]西北工业大学生命科学院,中国陕西西安710072 [2]中国科学院苏州生物医学工程技术研究所中国,江苏苏州215163

出　　处：《生命科学研究》2009年第4期320-326,共7页Life Science Research

摘　　要：磁场是一种普遍存在的环境因子,但其对微生物的影响尚不清楚.使用超导磁体产生的静态磁场,配以自制实验装置对所选菌株(枯草芽胞杆菌和鼠伤寒沙门氏菌)进行动态磁场处理,比较处理组与地磁场对照组之间包括生长曲线、酶活力等在内的各项生理生化指标,研究动态磁场对微生物的影响.研究发现,动态磁场下两株细菌的生长过程均受到影响.处理组枯草芽胞杆菌对数期提前,衰亡期菌体死亡速率受抑制,碱性蛋白酶活力增加,中性蛋白酶活力降低,而蛋白酶表达量无差异;鼠伤寒沙门氏菌动态磁场处理组对数期推迟,衰亡期菌体死亡亦受到抑制,细菌粘附实验与侵袭力实验表明,细菌毒力相对增强.以上结果表明,动态磁场可对微生物产生影响,并随着微生物种类的不同而异.Magnetic field is an ubiquitous environmental factor, but its impact on the micro-organisms is not clear. We employed dynamic magnetic field （DMF） by self-conducted apparatuses which based on the Superconducting Magnet（treat group） and the Geomagnetic field （control group） to dispose the mode strains （Bacillus subtilis and Salmonella typhimurium）, compared the physiological and biochemical indicators （including the growth curve, the enzyme activity etc.） between the two groups, to study the impact of dynamic magnetic field to these two microorganisms. The results showed that DMF affect the growth of both strains significantly. Logarithmic phase of treated Bacillus subtilis came earlier. In the decline phase, death rate of bacterial was restrained, alkaline protease activity increased, neutral proteinase activity decreased whereas protein expression showed no difference. The logarithmic phase of the treated Salmonella typhimurium was delayed. The death rate of bacterial was also restrained. Bacterial adhesion and invasion of the experiments revealed that the bacterial virulence enhanced relatively. The results above indicated that DMF has impact on bacteria, but the impact varies according to their types.

关 键 词：超导磁体 动态磁场 酶活力 侵袭力 粘附力 

分 类 号：S852.61[农业科学—基础兽医学] TS251.65[农业科学—兽医学]