血小板因子-4对人脑胶质瘤细胞增殖能力的影响  

Inhibitory effect of platelet factor-4 in the proliferation of human glioma cells

作　　者：曹春光[1] 谢方民[1] 杨明峰[2] 郑修启[1] 姬广福[1] 

机构地区：[1]泰山医学院附属医院,山东泰安271000 [2]泰山医学院生命科学研究所,山东泰安271000

出　　处：《济宁医学院学报》2009年第4期249-251,共3页Journal of Jining Medical University

摘　　要：目的探讨PF-4对人脑胶质瘤细胞增殖能力的影响。方法人脑胶质瘤细胞U251传代培养,分别加入浓度为0(对照组)、900ng/ml、1200ng/ml、1500ng/ml的PF-4。采用MTT比色法测定OD值;利用LSCM测定经Fluo-3标记的脑胶质瘤细胞内游离Ca2+荧光强度。结果1.对照组OD值为0.261±0.016;实验组OD值分别为0.210±0.008、0.193±0.010、0.162±0.019。经Student-Newman-Keul法比较,P<0.05。2.对照组脑胶质瘤细胞内游离Ca2+荧光强度为24.21±2.36;实验组脑胶质瘤细胞内游离Ca2+荧光强度分别为39.35±3.31、52.39±3.26、65.56±2.81。经单因素方差分析,各组间差异P<0.05。结论PF-4对人脑胶质瘤细胞增殖有明显的抑制作用,且具有剂量依赖性,随浓度的增加其抑制作用逐渐增强。Objective To discuss the inhibitive effect of PF-4 on the human gliomas. Methods Human gliomas （U251） were subcultured and the final concentration of Ong/ml, 900ng/ml, 1200ng/ml and 1500ng/ml of PF-4 were joined. The OD value was measured by MTT and intracellular free Ca2. fluorescence intensity of Fluo-3- labeled glioma cell was measured by LSCM.Results （ 1 ）The OD value of the control group and experimental groupwere 0. 261±0. 016; 0. 210±0. 008; 0. 193±0. 010;0. 162±0. 019. The OD value of each group were compared under Student-Newman-Keul method. There was a significance （P〈0.05）. （2）The human glioma cell intracellular free Ca2＋ fluorescence intensity of control group was 24.21 ± 2.36 ,after 24 hours, the value is respectively 39.35 ±3.31,52. 39±3.26 and 65.56±2.81. By one-way ANOVA,there was a significance. Conclusion PF-4 can inhibit the proliferation of human glioma cells and is dose- dependent.

关 键 词：脑胶质瘤 细胞增殖 血小板因子-4 

分 类 号：R739.41[医药卫生—肿瘤]