全人源抗结肠癌噬菌体单链抗体库的构建  

Construction of Human Anti-colon Carcinoma Single-chain Fv Fusion Phage Libraries

作　　者：谢平丽[1] 王红红[1] 李官成[2] 周国华[2] 李跃辉[2] 郭锋杰[2] 

机构地区：[1]中南大学护理学院,湖南长沙410013 [2]中南大学肿瘤研究所,湖南长沙410078

出　　处：《医学临床研究》2009年第8期1406-1409,共4页Journal of Clinical Research

摘　　要：【目的】构建全人源抗结肠癌噬菌体单链抗体库。【方法】采用体外致敏、EBV转化技术，PCR技术，噬菌体展示技术。【结果】构建了库容量达4×10^9的全人源抗结肠癌噬菌体ScFv库，ScFv基因的插入率为90％。【结论】体外致敏联合EBV转化及噬菌体展示技术制备抗结肠癌单链抗体库，这一策略是完全可行的，为进一步筛选抗结肠癌特异性单链抗体奠定了基础。[Objective]To construct human anti-colon carcinoma single-chain Fv（ScFv） fusion phage libraries. [Methods] Immunization in vitro, EBV transformation, PCR and phage display techniques were applied. [Results]A phage antibody library containing 4.0×10^9 different colon was obtained. The percentage of full-length ScFv gene inserted into phage DNA was 90%. [Conclusion]It is a feasible strategy for construct anti colon carcinoma cells ScFv antibody library by immunization in vitro, EBV transformation and phage display techniques. It provides a way for screening specific antibody against colon carcinoma.

关 键 词：结肠肿瘤 噬菌体 抗体 

分 类 号：R735.35[医药卫生—肿瘤]