浓香型大曲微生物群落结构的PCR-SSCP分析条件优化  

Optimal Conditions of PCR-SSCP Technology for the Analysis of Microbial Communities in Luzhou-flavor Daqu

作　　者：罗惠波[1] 李浩[1] 黄治国[1] 张强[1] 卫春会[1] 沈才萍[1] 

机构地区：[1]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000

出　　处：《四川理工学院学报（自然科学版）》2009年第4期72-74,共3页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

基　　金：四川省科技支撑项目(07GG013-004);四川省应用基础项目(2006J13-006);泸州老窖股份有限公司科研奖学金资助

摘　　要：文章研究了浓香型大曲微生物群落结构的PCR-SSCP分析条件的优化,结果表明:PCR产物100℃变性5 min后,在交联度为49:1,浓度为8%的聚丙烯酰胺凝胶中,无甘油,4℃条件下200 V电泳15 h,可得到较理想的图谱。The optimal conditions of PCR-SSCP Technology for the analysis of microbial communities in Luzhou-flavor Daqu is studied in the paper. The results show that under the condition of the concentration of the gel being 8%, the proportion of acrylamide and N, N＇-methylene-bisacrylamidei being 49： 1, without glycerine, denaturing at 100℃ for 5 min, 4℃, 200 V, electrophoresis 15 h, perfect spectrogram can be achieved.

关 键 词：PCR—SSCP 条件优化 微生物群落 

分 类 号：Q938.1[生物学—微生物学]