白介素-8在局灶性脑缺血大鼠脑组织及血清中的表达及地塞米松对其的影响  

Expression of interleukin-8 in the brain tissue and serum and the effect of Dexamethason in rats with local cerebral ischemic

作　　者：李霖[1] 李霞[2] 房绍宽[2] 刘亢丁[2] 

机构地区：[1]长春医学高等专科学校内科教研室,130031 [2]吉林大学第一医院神经内科

出　　处：《临床神经病学杂志》2009年第4期275-277,共3页Journal of Clinical Neurology

基　　金：国家自然科学基金资助项目(30770755)

摘　　要：目的探讨白介素(IL)-8在局灶性脑缺血大鼠脑组织及血清中的表达及地塞米松对其的影响。方法实验大鼠随机分为空白对照组、假手术组、缺血组及地塞米松组,后两组又各分为术后1h、2h、4h、8h、12h、24h、48h、72h组;每组12只。采用线栓法制备大鼠大脑中动脉栓塞模型;在相应的时间点应用ELISA法检测各组脑组织及血清中IL-8的水平。结果缺血组与地塞米松组脑组织及血清中IL-8水平于缺血1h后逐渐升高,后于24h及4h达高峰;缺血组与地塞米松组脑组织及血清中IL-8水平各时间点又低于空白对照组与假手术组(均P<0.05),地塞米松组又低于缺血组(均P<0.05)。结论局灶性脑缺血大鼠脑组织及血清中IL-8表达与时间关系密切;地塞米松可明显抑制局灶性脑缺血大鼠脑组织中炎性反应。Objective To explore the expression of interleukin - 8 （ IL-8 ） in the brain tissue and serum and effect of Dexamethason in rats with local cerebral ischemic. Methods The rats were randomly divided into blank control group, shamoperated group, ischemic group and Dexamethasone group. The latter two groups were divided into 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h, 72 h after oppration groups,respectively, 12 rats in each group. The cerebral ischemical models were made by thread-embolism. The expression of IL-8 in brain tissue and serum at each time point were detected by ELISA method. Results In the ischemic group and Dexamethasone group, the expression of IL-8 of the brain tissue and serum were increased gradually in 1 h after ischemia, and reached the perk in 24 h and 4 h respectively. Which were significantly lower than those in the blank control group or shamoperated group（ all P 〈 0. 05 ） at each time point. Also, with in Dexamethasone grpup was remarkable lower than those in ischemie group（ all P 〈 0. 05 ）. Conclusions The expression of IL-8 in the brain tissue and serum are closely correlated with the time after the model made in the rats with focal cerebral ischemical. Dexamethasone can obviously inhibit inflammatory reaction of brain tissue in focal cerebral ischemic rat.

关 键 词：局灶性脑缺血 白介素-8 地塞米松 

分 类 号：R743.3[医药卫生—神经病学与精神病学]