杏仁核电点燃及海人酸点燃难治性癫大鼠脑组织及外周血P糖蛋白的表达  

Expression of P-glycoprotein in brain tissue and peripheral blood in refractory epileptic rats with amygdale kindled by electricity and kainic acid

作　　者：杨春晓[1] 杨跃[1] 李晋峰[1] 金永华[1] 梁庆成[1] 

机构地区：[1]哈尔滨医科大学附属第二医院神经内科,150086

出　　处：《临床神经病学杂志》2009年第4期289-292,共4页Journal of Clinical Neurology

基　　金：黑龙江省博士后基金项目(LRB05-726)

摘　　要：目的探讨杏仁核电点燃及海人酸点燃难治性癫大鼠模型脑组织及外周血P糖蛋白(Pgp)过度表达的可能机制,并比较不同方法致难治性癫大鼠脑组织及外周血中Pgp的表达趋势。方法应用电刺激点燃及海人酸点燃方法对大鼠杏仁核进行点燃,制作难治性癫模型;将其分为A组(杏仁核电点燃,12只),B组(杏仁核海人酸点燃,12只),另设C组(杏仁核埋入电极而不点燃,且给予卡马西平灌胃,8只),D组(杏仁核注射生理盐水,且给予卡马西平灌胃,8只),E组(杏仁核埋入电极而不点燃,且给予生理盐水灌胃,8只),F组(杏仁核注射生理盐水,且给予生理盐水灌胃,8只);采用免疫组化方法分析两种模型大鼠脑组织及外周血Pgp的表达。结果A组、B组大鼠脑组织及外周血Pgp表达明显高于其余4组,差异有统计学意义(均P<0.05);C组、D组大鼠脑组织及外周血Pgp表达高于E组及F组,差异有统计学意义(均P<0.05);A组与B组大鼠脑组织及外周血Pgp的表达差异无统计学意义。结论脑组织及外周血Pgp的过度表达可能是未能控制的癫发作、服用抗癫药(AEDs)等多因素共同作用的结果;难治性癫发生1个月后,脑组织中Pgp高表达的大鼠外周血中Pgp的表达同样高于脑组织中Pgp低表达大鼠外周血Pgp的表达,提示难治性癫大鼠外周血及脑组织中Pgp的表达趋势具有一定的相关性。Objective To explore the possible mechanism of over expression of P-glycoprotein （Pgp） in brain tissue and peripheral blood in refractory epileptic rats with amygdale kindled by electricity and kainic acid, and compare the expression tendency of Pgp in brain tissue and peripheral blood of the different ways to establish refractory epileptic rats models. Methods The models of refractory epileptic rats were made by electrical kindling and kainic kindling in amygdale. Group A was for amygdale-electrical-kindling rats （ n = 12 ）, group B was for amygdale-kainic-kindling rats （n = 12）, group C was implanted electrode without kindling and Carbamazepine was given by gavage（n = 8）, group D was injected saline in amygdale and Carbamazepine was given by gavage （n = 8）, group E was implanted electrode without kindling and saline was given by garage（ n = 8 ） and group F was injected saline in amygdale and saline was given by gavage（ n = 8 ）. The expression of Pgp in brain tissue and peripheral blood was detected by immunohistochemistry in each group. Results The expression of Pgp in brain tissue and peripheral blood of rats in group A and B were both much higher than those in the other groups and there were statistical differences （ all P 〈 0.05 ）. The expressions of Pgp in brain tissue and peripheral blood of rats in group C and D were higher than those in group E and group F. There were statistical differences （ all P 〈 0. 05 ）. There were no statistical difference in the expression of Pgp in brain tissue and peripheral blood between group A and group B. Conclusions The over expression of Pgp in brain tissue and peripheral blood may be multiple factors combined effect result of uncontrolled epilepsy and antiei^ileptic drugs. The expression of Pgp in peripheral blood in the rats with brain tissue high expression of Pgp is higher than that in the rats with brain tissue lower expression of Pgp after refractory epilepsy onset 1 mon. Therefore, there is correlation of the expression tende

关 键 词：难治性癫痫 P糖蛋白 杏仁核 电刺激点燃 海人酸点燃 

分 类 号：R742.1[医药卫生—神经病学与精神病学]