蕈样肉芽肿皮肤冰冻组织中T细胞受体-Vγ基因重排的检测  

Detection of T cell receptor gene rearrangement in the frozen cutaneous tissue of mycosis fungoides

作　　者：郭英军[1,2] 郭春芳[3] 王雅坤[1] 金光玉[1] 赵玉铭[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科,沈阳110001 [2]中国医科大学附属盛京医院皮肤科,沈阳110004 [3]沈阳市第七人民医院

出　　处：《中国麻风皮肤病杂志》2009年第8期567-569,共3页China Journal of Leprosy and Skin Diseases

基　　金：辽宁省教育厅资助课题(编号:05L575)

摘　　要：目的:评价蕈样肉芽肿(MF)冰冻组织基因组DNA T细胞抗原受体(TCR)Vγ基因克隆性重排法对MF基因诊断中的价值。方法:一步法提取37例MF患者冰冻组织基因组DNA,用两套Vγ1-8/Jγ和Vγ9/Jγ基因重排的引物进行两次PCR扩增。结果:在30例MF中Vγ1-8/Jγ引物检出420 bp条带TCR-γ基因克隆性重排;在28例MF中Vγ9/Jγ引物检出380 bp条带TCR-γ基因克隆性重排;同时出现Vγ1-8/Jγ和Vγ9/Jγ TCR-γ基因重排的有26例,阳性率为86.49%。结论:MF冰冻组织中T细胞受体γ基因的重排在诊断中简便快速、符合率较高;检测方法具有较高的灵敏度。Objective： To evaluate the value of rearrangement of T cell antigen receptor （TCR） -γ in the frozen tissue in the genetic diagnosis of mycosis fungoides （MF）. Methods： Genomic DNA was extracted from frozen tissue of 37 MF patients by one - step method. Two sets of Vγ1 - 8/Jγ and Vγ9/Jγ rearrangement primers were designed. Two rounds of polymerase chain reaction （PCR） were applied. Result： TCR- γ gene rearrangement at the 420 bp band was detected by Vγ1 - 8 and Jγ primer in 30 patients, at the 380 bp band detected by Vγ9 and Jγ primer in 28 patients, and detected by both Vγ1 - 8/Jγ and Vγ9/Jγ primers in 26 patients, with a positivity rate of 86.49%. Conclusion： TCR- γ gene rearrangement method is a simple and convenient with high sensitivity in the diagnosis of MF.

关 键 词：聚合酶链反应 T细胞抗原受体 基因重排 

分 类 号：R739.5[医药卫生—肿瘤] R733.71[医药卫生—临床医学]