二氢青蒿素对人宫颈癌HeLa细胞放射增敏作用的研究  

Radiation sensitization by dihydroartemisinin on human HeLa cells of cervical cancer

作　　者：陈遐林[1] 曹建平[1] 纪蓉[1] 朱巍[1] 刘杨[1] 宫晓梅[1] 唐艳[1] 潘春燕[1] 樊赛军[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院江苏省放射医学与防护重点实验室,215123

出　　处：《中华放射医学与防护杂志》2009年第4期360-363,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：基金项目：国家自然科学基金（30670632）

摘　　要：目的探讨二氢青蒿素对人宫颈癌HeLa细胞的放射增敏作用。方法用MTT法检测其对HeLa细胞生长的抑制效应；克隆形成法检测放射敏感性；应用流式细胞仪检测细胞周期及细胞凋亡的变化。结果二氢青蒿素对HeLa细胞有明显的抑制作用，其效应呈时间和剂量依赖性。20μmol／L二氢青蒿素对HeLa细胞的放射增敏比（SER）为1．47。二氢青蒿素能够去除x线导致的HeLa细胞G2期阻滞，与单纯6Gy照射组相比，药物＋照射组G2期阻滞由73．58％降至48．31％。二氢青蒿素能增强X线诱导的细胞凋亡，与单纯2、4、6Gy照射组相比，药物＋照射组凋亡率分别由29．46％、48．04％、70．21％升至45．79％、66．36％、79．58％。结论二氢青蒿素对人宫颈癌HeLa细胞具有放射增敏作用，机制可能与二氢青蒿素去除照射引起的G2期阻滞有关。Objective To investigate the radiosensitizing effects of dihydroartemisinin （DHA）on human HeLa ceils of cervical cancer irradiated by X rays. Methods Cell growth kinetics was determined using MTr assay. Cell survival was analyzed by clonogenic assay. The change of cell cycle and apoptosis was measured by flow cytometry. Results Dihydroartemisinin inhibited the growth of HeLa cells of human cervical cancer and showed a dose-dependent and time-dependent manner. Dihydroartemisinin （20 μmol/L） showed the radiosensitizing effects on HeLa cells, and the sensitizing enhancement ratio （SER） was 1.47. Dihydroartemisinin abrogated radiation-induced G2 arrest of the tested HeLa cells, the G2 ratio of medicine ＋ radiation group declined from 73.58% to 48.31%. Dihydroartemisinin enhanced the apoptosis of HeLa cells by X-irradiation, the apoptosis rates of medicine ＋ radiation group significantly increased from 29.46%, 48.04%, 70.21% to 45.79%, 66.36% and 79.58%, respectively for 2, 4 and 6 Gy. Conclusions Dihydroartemisln/n could increase the radiosensitivity of HeLa cells of human cervical cancer. Abrogation of radiation-induced G2 arrest could be part of the mechanism.

关 键 词：二氢青蒿素 人宫颈癌细胞 放射增敏 细胞周期 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]