牵张力与雌激素对兔鼻软骨细胞增殖效应调节的初步研究  

Effects of Distraction and Estradiol on the Proliferation of Rabbit Nasal Chondrocytes in Vitro

作　　者：白明海[1] 吴汉江[1] 

机构地区：[1]中南大学湘雅二医院口腔科,湖南长沙410011

出　　处：《口腔医学研究》2009年第4期412-415,共4页Journal of Oral Science Research

基　　金：湖南省科技厅基金资助项目(编号:1JZY2017)

摘　　要：目的：探讨牵张力与雌激素对兔鼻软骨细胞增殖效应的影响。方法：1）采用MTT四唑盐比色法,检测不同剂量的17~β雌二醇（E2）（10^-12-10^-6mol/L）对第4代新生及6周龄新西兰白兔兔鼻软骨细胞增殖活性的影响。2）将第4代新生及6周龄新西兰白兔兔鼻软骨细胞在细胞膜式牵张力施加装置上培养,检测不同浓度17-β雌二醇（10^-10-10^-8mol/L）及5 kPa牵张力联合应用0、12 h后,对兔鼻软骨细胞增殖活性（PI值）的影响。结果：（1）10^-10-10^-8mol/L17-β雌二醇体外培养新西兰白兔兔鼻软骨细胞生长具有刺激作用,且呈剂量依赖性,最大效应浓度为10^-8mol/L,进一步增加E2浓度则刺激作用减弱。10^-6mol/L具有明显抑制性。（2）5 kPa牵张力联合17-β雌二醇培养较17-β雌二醇的单独作用具有更强的促兔鼻软骨细胞增殖作用。结论：牵张力与雌激素可以调节兔鼻软骨细胞增殖活性。Objective： To investigate to effects of static tension-stress and estradiol on the proliferation of rabbit nasal chondrocytes in vitro.Methods： 1） The fourth-passage chondrocytes were harvested from the nase of neonatal and 6-week-old rabbits.Estradiol（E2） was added into the culture medium at a variety of concentrations.Cell growth was measured by using MTT method in order to investigate the effects of E2 on the proliferation of nasal chondrocytes in rabbits.2） The fourth-passage chondrocytes were harvested from the nase of neonatal and 6 - week - old rabbits. The experiment specimens were divided into tour groups under continuous static distraction （5KPa） and different Estradiol （E2 ） dosage （ 10^-10 - 10 ^-8mol/1） for O and, 12 hours. Muhivariable analyses were conducted to test the associations between the proliferation of nasal chondrocytes among different Estradiol （ E2 ） dosages and different continuous static distractions. Results： 1 ） The results showed that E2 increased cell proliferation at 10^-10 to 10^-8 mol/L in a dose -dependent manner,peaking at 10^-8 mol/1. However,further increased concentrations of E2 caused inhibition and the significance was at 10^-6 mol/1. The proliferation of cells in the neonatal rabbit group was more obvious than in the 6 - week - old rabbit group （ P 〈 0.05 ）. （2） The results showed that the most active mitogenesis happened in the group with continuous static distraction （5KPa） combined with Estradiol（ E2 ）. The proliferation of cells in the neonatal rabbit group was more obvious than in the 6 - week - old rabbit group （ P 〈 0.05 ）. Conclusion： The static tension - stress and estradiol can regulate the proliferation of rabbit nasal chondrocytes in vitro.

关 键 词：牵张力 雌激素 鼻软骨细胞 增殖活性 

分 类 号：R782[医药卫生—口腔医学] R765[医药卫生—临床医学]