牙本质非胶原蛋白诱导牙髓-牙本质复合体反应的动物实验观察  

Experimental Study on the Response of Dentine-pulp Complex Inducing by Dentin Non-collagenous Proteins

作　　者：李俊[1] 李政权[1] 高原[1] 

机构地区：[1]深圳市第三人民医院口腔科,广东深圳518020

出　　处：《口腔医学研究》2009年第4期439-441,共3页Journal of Oral Science Research

摘　　要：目的:观察提取的猪牙本质非胶原蛋白对修复性牙本质形成的诱导活性。方法:用提取的猪EDTA可溶性牙本质非胶原蛋白作盖髓剂,对Wistar大鼠的健康第一磨牙进行直接盖髓实验,采用氢氧化钙(Dycal)和空白组为对照,通过组织学实验观察牙髓-牙本质复合体的反应。结果:盖髓术后7 d,各组之间软、硬组织反应的差异无统计学意义。术后14 d,NCPs组和Dycal组的修复反应均明显优于空白对照组(P<0.01),且NCPs组对牙髓的刺激小于Dycal组(P<0.05)。结论:NCPs对牙髓刺激性小,可以诱导牙髓细胞分化为成牙本质细胞样细胞,形成修复性牙本质,其诱导活性至少与常规盖髓剂相当。Objective： To observe the induction effects of dentin non-collagenous proteins（NCPs） extracted from pig teeth on reparative dentinogenesis.Methods： Direct pulp capping with isolated dentin NCPs was performed in the first molars of Wistar rats.Dycal and no medication were used as the control.The response of dentine-pulp complex was observed at 7 and 14 days after surgery.Results： 7 days After operation,standardized grading of the soft and hard tissue responses revealed no statistical significant differences among the treatment groups. After 14 days, NCPs samples and Dyeal samples showed improved soft and hard tissue repair when compared with the control. At this time point, samples from the control group displayed severe inflammation and Dycaltreated samples showed diffuse intrapulpal inflammation,whereas NCPs- treated samples exhibited mild inflammation localized to the exposure site only. Conclusion： The study indicate that the application of NCPs into the injured pulp may limit the inflammatory response, stimulate pulpal progenitor cells to differentiate into odontoblast - like cells which are responsible for the formation of reparative dentine. The distinguishing outcome of NCPs treatment suggest its potential as a pulp -capping medication.

关 键 词：牙本质非胶原蛋白 直接盖髓术 修复性牙本质 

分 类 号：R781.05[医药卫生—口腔医学]