磁性纳米四氧化三铁协同顺铂作用于肺癌A549细胞的初步研究  

Synergistic effect of magnetic nanopartical Fe_3O_4 with cisplatin on pulmonary adenocarcinoma cell A549

作　　者：周颐[1] 姜藻[1] 顾晓怡[1] 

机构地区：[1]东南大学附属中大医院肿瘤中心,江苏南京210009

出　　处：《东南大学学报（医学版）》2009年第4期297-301,共5页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:检测磁性纳米四氧化三铁(Nano-Fe3O4)联合顺铂(DDP)作用于人源肺腺癌细胞A549后相关凋亡抑制基因、耐药蛋白的表达,研究其增强化疗敏感度的机制。方法:用MTT法检测Nano-Fe3O4聚合DDP对A549细胞增殖凋亡的影响;流式细胞术分析凋亡细胞比例;RT-PCR检测抗凋亡基因bcl-2、survivin、ERCC1表达水平变化;蛋白印迹法分析耐药蛋白MRP及其表达率。结果:25μg.ml-1Nano-Fe3O4能有效增强DDP的细胞毒作用,提高A549的凋亡率;与单独用药组相比,Nano-Fe3O4联合DDP组的抗凋亡基因bcl-2、ERCC1和耐药蛋白MRP表达下调,差异具有统计学意义(P<0.05);survivin基因表达未见明显改变。结论:Nano-Fe3O4联合DDP可通过下调抗凋亡基因bcl-2、ERCC1及耐药蛋白MRP的表达,增强化疗药物敏感度。Objective To study the effect of magnetic nanoparticalFe3O4 with cisplatin on the expression level of apoptosis-related genes and drug resistance protein in pulmonary adenocarcinoma cell A549 and to explore the different mechanisms in increasing the therapeutic effectiveness. Methods The inhibitory effects of magnetic Nano-Fe3O4 and cisplatin on the proliferation A549 cell line were evaluated by MTT assay. Cell apoptosis was detected by flow cytometry. The changes of bcl-2 ,survivin and ERCC1 mRNA were quantified by RT-PCR. The expressive rates of multi-drug resist- ant-associated protein MRP were quantified by Western blotting. Results 25 μg. ml-1 Nano-Fe3O4 with cisplatin in- duced more apoptosis percentage. Compared to the control, Nano-Fe3O4 or DDP treatment group,there existed a decrease in bcl-2 and ERCC1 mRNA expression in the combination group ,while survivin remained unchanged. And a decrease in the expression of muhidrug resistance associated protein MRP was observed. Conclusion The Nano-Fe3O4 with cisplatin significantly increases the therapeutic effectiveness in A549 cells by inducing more apoptosis and decreasing the expres- sion of bel-2,ERCC1 mRNA and MRP.

关 键 词：磁性纳米四氧化三铁 顺铂 肺腺癌细胞系A549 凋亡 多药耐药 

分 类 号：R734.2[医药卫生—肿瘤] R979.1[医药卫生—临床医学]