罗格列酮延缓糖尿病大鼠血管钙化的发展  

Effect of rosiglitazone on aorta calcification of diabetic rats

作　　者：谈君[1] 任晓妹[1] 刘乃丰[1,2] 

机构地区：[1]东南大学临床医学院心血管病研究所,江苏南京210009 [2]东南大学附属中大医院心内科,江苏南京210009

出　　处：《东南大学学报（医学版）》2009年第4期317-321,共5页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:观察罗格列酮对糖尿病大鼠钙化血管的治疗作用及对骨转录因子Msx2表达的影响。方法:在高脂饮食加小剂量链脲佐菌素腹腔注射处理的基础上,加用维生素D3和尼古丁处理,制备糖尿病合并血管钙化大鼠模型(糖尿病合并血管钙化组),给予部分糖尿病合并血管钙化组大鼠罗格列酮灌胃(2 mg.kg-1,每天1次,共8周)处理(罗格列酮组),同时设立空白对照组。实验第21周末,处死各实验组大鼠,检测各组大鼠的代谢指标,进行血管von Kossa染色,检测血管钙含量和碱性磷酸酶活性,应用real-time PCR方法检测Msx2 mRNA及蛋白免疫印迹方法检测Msx2蛋白在血管中的表达变化。结果:与对照组相比,糖尿病合并血管钙化组大鼠血管内存在弥漫性的钙盐沉积,血管内钙含量增高,碱性磷酸酶活性增强,Msx2 mRNA和蛋白表达明显增高(均P<0.05);而给予罗格列酮处理后,钙盐在血管内的沉积明显减少,钙含量和碱性磷酸酶活性分别下降8.37%、10.59%,Msx2 mRNA和蛋白表达也分别下降36.73%、42.55%(与糖尿病合并血管钙化组相比,均P<0.05)。结论:罗格列酮可以延缓糖尿病大鼠血管钙化的发展,并可以减少钙化血管中Msx2的表达。Objective To investigate the effect of rosiglitazone on aorta calcification of diabetic rats, and the ex- pression of Msx2 in calcified artery of diabetic rats. Methods Male Wistar rats were received a single low dose of strep- tozotocin （STZ） after 8 weeks of a high fat diet to induce diabetes, and then treated with vitamin D3 and nicotine （ DM ＋ VDN group）. Half of them were received rosiglitazone （2 mg＂ kg - 1 per day for 8 weeks, RSG group）. Measurements of metabolic parameters ,yon Kossa calcium stain, aortic calcium content and alkaline phosphatase （ALP） activity, real-time PCR for Msx2 mRNA, and western blotting for Msx2 protein were performed. Results In the DM ＋ VDN group, calcium deposition in aorta, aortic calcium content, ALP activity and expression of Msx2 mRNA and protein in calcified artery in- creased significantly as compared to control group （P 〈 O. 05 ）. 8 weeks of rosiglitazone treatment decreased the calcium deposition in aorta, respectively decreasing aortic calcium content and ALP activity by 8.37 %, 10.59 %. The expres- sion of Msx2 mRNA and protein in calcified artery were also decreased by 36.73 % ,42.55 % compared to DM ＋ VDN group （P 〈 0.05）. Conclusions Long-term rosiglitazone treatment can attenuate the effect of diabetes on rat vascular calcification, and down-regulate the expression of Msx2 mRNA and protein in calcified artery.

关 键 词：2型糖尿病 血管钙化 转录因子Msx2 罗格列酮 大鼠 

分 类 号：R-33[医药卫生] R587.1