慢性阴塞性肺疾病大鼠模型体内TNFα及P65含量变化及意义  

Varying Contents of TNF α and P65 in Rat Models of Chronic Obstructive Pulmonary Disease and its Significance

作　　者：胡蓉[1] 黄燕[1] 姜虹[1,2] 朱也森[1] 严国锋[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院麻醉科,上海200011 [2]上海交通大学医学院实验动物科学部,上海200025

出　　处：《中国医药导刊》2009年第8期1336-1338,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:探讨TNFα及P65在慢性阻塞性肺病(COPD)大鼠肺组织中的含量变化及其在发病中的作用。方法:香烟熏吸复合脂多糖(LPS)气道内滴注法复制COPD模型。将20只雄性清洁级Sprague-Dawley大鼠随机分为对照组和COPD模型组,每组各10只。肺组织切片行苏木精-伊红染色,测定两组支气管肺泡灌洗液(BALF)中TNFα含量,免疫组化法测定两组大鼠气道上皮中P65表达,运用Image-Proplus6.0软件行半定量分析。结果:模型组大鼠BALF中TNFα及肺组织中P65表达较对照组明显升高(P<0.05)。结论:TNFα及P65参与了大鼠COPD的发病过程且在炎症反应中起重要作用。Objective： To discuss the varying contents of TNFα and P65 and their effect on experimental rat model of chronic obstructive pulmonary disease. Methods： The rat models of COPD was established by passive smoking and endotracheal instillation of LPS. 20 male Sprague- Dawley rats were randomly divided into two groups; Control and COPD model group, 10 rats each. The slides of lung tissue were dyed with Hematoxylin-Eosin. The concentration of TNF ct was tested in BALF. The expression of P65 was tested by immnohistochemistry. Software Image- Proplus6.0 was applied to do their semi-quantitative analysis. Results： The concentration ofTNF α in BALF in COPD group was much higher than that in control group （P〈0.05）.The expression of P65 in COPD group was much higher than that in control group （P〈0.05）. Conclusion： TNFα and P65 took part in the pathological progress of COPD and played important role in inflammation reaction.

关 键 词：慢性阻塞性肺病 P65 TNFΑ 炎症反应 

分 类 号：R563.9[医药卫生—呼吸系统]