丹参酮ⅡA对肺缺血/再灌注损伤大鼠的保护作用  

Protective Effects of TanshinoneⅡA on Ischemia-reperfusion Lung Iniury in Rats

作　　者：李钰娜[1] 

机构地区：[1]长治医学院生物化学教研室,046000

出　　处：《长治医学院学报》2009年第4期257-259,共3页Journal of Changzhi Medical College

摘　　要：目的:探讨丹参酮ⅡA(TanshinoneⅡA,TanⅡA)对大鼠肺缺血/再灌注损伤的保护作用及机制。方法:将40只SD大鼠随机分为四组:对照组、模型(I/R)组、低剂量干预组、高剂量干预组,每组10只。模型组和干预组用血管夹夹闭左侧肺门30 min,去除血管夹恢复血流2 h。干预组于缺血前3 d给予不同剂量丹参酮ⅡA灌胃。对照组和模型组灌服等量生理盐水。测定各组肺组织的湿干比(W/D)、肺组织中髓过氧化物酶(MPO)活性、丙二醛(MDA)的含量。结果:模型组MPO、MDA、以及W/D较对照组显著增高。干预组各项指标均明显低于模型组,高剂量干预组各项指标均明显低于低剂量干预组。结论:TanⅡA对肺缺血/再灌注损伤大鼠有显著的保护作用,其机制可能与抗氧化自由基损伤、抑制中性粒细胞激活有关。Objective： To investigate protective effects of Tanshinone ⅡA （Tan Ⅱ A） on isehemia - reperfusion lung iniury in rats and the possible mechanisms. Methods：40 SD rats were randomly divided into 4 groups： control group, model group（I/R）, low dose Tan Ⅱ A group and high dose Tan Ⅱ A group. Tan ⅡA were administered to rats in experimental groups 3 days before test, while physiological saline were administered to rats in control and model groups at the same time. Isehemia - reperfusion injury were indueed by ligation of left pulmonary artery. Ratio of wet to drying （W/D）, malondialdehyde （MDA） and myeloperoxidase （MPO） were determined. Results： W/D, MDA and MPO in model group were significantly higher than those in control group. Compared with W/D, MDA and MPO in model group, those in experimental group were significantly lower. Conclusion ： Tan Ⅱ A protects lung from isehemia - reperfusion iniury in rats. The mechanism is related to its antioxidative effect and the inhibition of neutrophil activation.

关 键 词：丹参酮ⅡA 肺 再灌注损伤 

分 类 号：R364[医药卫生—病理学]