拟南芥莲座叶芥子油苷含量对水分胁迫的响应  

Water stress on glucosinolate contents in Arabidopsis rosette leaves

作　　者：任欢[1] 钟海秀[1] 戴绍军[1] 陈思学[2] 阎秀峰[1] 

机构地区：[1]东北林业大学生命科学学院,东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040 [2]佛罗里达大学植物学系,盖恩斯维尔FL32611,USA

出　　处：《生态学报》2009年第8期4372-4379,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金海外青年学者合作研究基金资助项目(30528013);国家自然科学基金资助项目(30670325);国家新世纪优秀人才支持计划资助项目(NCET-05-0328)

摘　　要：芥子油苷(glucosinolates)是十字花科植物中一类含氮、含硫的次生代谢产物,与其水解产物在植物防御功能中有重要意义且与环境因子关系密切。通过控制供水的方式对营养生长时期的拟南芥幼苗进行水分胁迫,观察了土壤自然干旱对营养生长时期拟南芥莲座叶芥子油苷含量及组成的影响。结果表明,土壤自然干旱处理下,拟南芥莲座叶的芥子油苷总量从处理3 d起低于对照,且随着处理天数的增加与对照组的差异逐渐增大,脂肪族芥子油苷的响应均比较明显,与芥子油苷总量的变化趋势基本一致,而吲哚族芥子油苷对水分胁迫则不敏感。脂肪族中的4-甲基亚磺酰丁基芥子油苷(4-methylsulphinylbutyl GS,4MSOB)占脂肪族芥子油苷的比例最大,它的含量变化成为影响莲座叶中芥子油苷组合模式的主导因素。Glucosinolates are nitrogen-and sulfur-containing specialized metabolites found in the plant order Capparales,which includes cruciferous plants.Glucosinolates and their degradation products play important roles in plant defense and in interactions with the environment.Here we report the effect of soil water stress on glucosinolate content in rosette leaves of Arabidopsis thaliana during the vegetative stage.The results showed that the water stress treatment caused a significant reduction in glucosinolate levels in rosette leaves of A.thaliana compared to well-irrigated control samples after 3 days of treatment.The differences between control and treatment became increasingly more significant over time,with both total and aliphatic glucosinolate content affected.Indole glucosinolates did not exhibit significant response to water stress.The content of 4-methylsulphinylbutyl glucosinolate（4MSOB） in rosette leaves was the highest among all the aliphatic glucosinolates detected.The decrease in the contents of 4MSOB after water stress is a main factor that affected the overall glucosinolate profile in the rosette leaves.

关 键 词：拟南芥 芥子油苷 水分胁迫 

分 类 号：Q945.78[生物学—植物学]