波吉卵囊藻对抗生素的敏感性及其无菌化培养  

Sensitivity to Antibiotics and Axenic Culture of Oocystis borgei

作　　者：李静红[1] 黄翔鹄[1] 刘慧玲[1] 

机构地区：[1]广东海洋大学水产学院,广东湛江524025

出　　处：《广东海洋大学学报》2009年第3期37-41,共5页Journal of Guangdong Ocean University

基　　金：广东省科技计划项目(2006A20305003);广东省自然科学基金(8152408801000003)

摘　　要：研究了青霉素、链霉素、庆大霉素和卡那霉素对波吉卵囊藻生长的影响,采用4种方法添加抗生素获得无菌藻株,比较波吉卵囊藻除菌前后的生长特点。结果表明:青霉素和链霉素浓度为1000IU/mL时,对波吉卵囊藻生长无明显影响;庆大霉素和卡那霉素浓度达500IU/mL时,对波吉卵囊藻生长无明显影响。微藻培养液中细菌对抗生素敏感性实验表明,除青霉素外,链霉素、庆大霉素和卡那霉素均对藻液中细菌有明显抑制作用。采用4种方法添加抗生素,均可获得无菌藻株。与未除菌时相比,无菌的波吉卵囊藻不易老化。Effects of 4 kinds of antibiotics （penicillin, streptomycin, kanamycin, gentamycin） on the growth of Oocystis borgei were studied. The axenic Oocystis borgei was obtained by four ways of adding antibiotics, and the characteristics of growth between nonaxenic and axenic Oocystis borgei were compared as well. The results showed： the growth of Oocystis borgei was not significantly affected by penicillin and streptomycin at the concentration of 1 000 IU/mL, and there were no significant effect on the growth of this alga when the concentration of kanamycin and gentamycin were 500 IU/mL. Except penicillin, there were bacteriostasis effects of the other 3 kinds of antibiotics. Antibiotics were added to the culture medium by 4 processes, and the axenic culture could all be obtained. Compared with axenic Oocystis borgei, the nonaxenic algae aged faster.

关 键 词：波吉卵囊藻 抗生素 敏感性 无菌化培养 

分 类 号：X173[环境科学与工程—环境科学]